Rabbit anti-Human PSG7 Polyclonal Antibody | anti-PSG7 antibody

PSG7, CT (PSG7, Putative pregnancy-specific beta-1-glycoprotein 7) (APC)

Cat. Num. AAA6338096

• Gene Names: PSG7; PSG1; PSGGA; PSBG-7
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSG7; Polyclonal Antibody; CT (PSG7; Putative pregnancy-specific beta-1-glycoprotein 7) (APC); Putative pregnancy-specific beta-1-glycoprotein 7; anti-PSG7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PSG7 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PSG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 270-300 from the C-terminal region of human PSG7.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PSG7 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). PSG7 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(PSG7 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PSG7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PSG7 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PSG7 antibody

PSG7 is a member of the pregnancy-specific glycoprotein (PSG) gene family. The PSG genes are a subgroup of the carcinoembryonic antigen (CEA) family of immunoglobulin-like genes, and are found in a gene cluster at 19q13.1-q13.2 telomeric to another cluster of CEA-related genes. The PSG genes are expressed by placental trophoblasts and released into the maternal circulation during pregnancy, and are thought to be essential for maintenance of normal pregnancy. The reference genome contains a nonsense mutation that disrupts the coding sequence, suggesting that this gene may be evolving into a pseudogene.

Product Categories/Family for anti-PSG7 antibody

Antibodies; Carbohydrates; Glycoproteins

References

Grimwood, J., et al. Nature 428(6982):529-535(2004). Beauchemin, N., et al. Exp. Cell Res. 252(2):243-249(1999). Adams, M.D., et al. Nature 377 (6547 SUPPL), 3-174 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 157805480
• NCBI GeneID: 5676
• NCBI Accession #: NP_002774.2
• NCBI GenBank Nucleotide #: NM_002783.2
• UniProt Accession #: Q13046; Q15232
• Molecular Weight: 47,001 Da
• NCBI Official Full Name: putative pregnancy-specific beta-1-glycoprotein 7 isoform 1
• NCBI Official Synonym Full Names: pregnancy specific beta-1-glycoprotein 7 (gene/pseudogene)
• NCBI Official Symbol: PSG7
• NCBI Official Synonym Symbols: PSG1; PSGGA; PSBG-7
• NCBI Protein Information: putative pregnancy-specific beta-1-glycoprotein 7
• UniProt Protein Name: Putative pregnancy-specific beta-1-glycoprotein 7
• Protein Family: Putative pregnancy-specific beta-1-glycoprotein
• UniProt Gene Name: PSG7
• UniProt Synonym Gene Names: PS-beta-G-7; PSBG-7; Pregnancy-specific glycoprotein 7

NCBI Description

This gene is a member of the pregnancy-specific glycoprotein (PSG) gene family. The PSG genes are a subgroup of the carcinoembryonic antigen (CEA) family of immunoglobulin-like genes, and are found in a gene cluster at 19q13.1-q13.2 telomeric to another cluster of CEA-related genes. The PSG genes are expressed by placental trophoblasts and released into the maternal circulation during pregnancy, and are thought to be essential for maintenance of normal pregnancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

PSG7: a member of the pregnancy-specific glycoprotein (PSG) gene family. The PSG genes are a subgroup of the carcinoembryonic antigen (CEA) family of immunoglobulin-like genes, and are found in a gene cluster at 19q13.1-q13.2 telomeric to another cluster of CEA-related genes. The PSG genes are expressed by placental trophoblasts and released into the maternal circulation during pregnancy, and are thought to be essential for maintenance of normal pregnancy. The reference genome contains a nonsense mutation that disrupts the coding sequence, suggesting that this gene may be evolving into a pseudogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2011]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.31

Cellular Component: extracellular region

Biological Process: female pregnancy