Rabbit anti-Human PSAPL1 Polyclonal Antibody | anti-PSAPL1 antibody

PSAPL1, NT (PSAPL1, Proactivator polypeptide-like 1, Saposin A-like, Saposin B-Val-like, Saposin B-like, Saposin C-like, Saposin D-like) (PE)

Cat. Num. AAA6338039

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSAPL1; Polyclonal Antibody; NT (PSAPL1; Proactivator polypeptide-like 1; Saposin A-like; Saposin B-Val-like; Saposin B-like; Saposin C-like; Saposin D-like) (PE); Saposin D-like; anti-PSAPL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PSAPL1 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PSAPL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-166 amino acids from the N-terminal region of human PSAPL1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PSAPL1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PSAPL1 antibody detected the PSAPL1 protein (arrow).)

Flow Cytometry (FC/FACS)

(PSAPL1 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PSAPL1 antibody

PSAPL1 may activate the lysosomal degradation of sphingolipids (By similarity).

Product Categories/Family for anti-PSAPL1 antibody

Antibodies; Proteins

References

Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 145977198
• NCBI GeneID: 768239
• NCBI Accession #: NP_001078851.1
• NCBI GenBank Nucleotide #: NM_001085382.1
• UniProt Accession #: Q6NUJ1; Q8N7T4; A0A184
• Molecular Weight: 56,627 Da
• NCBI Official Full Name: proactivator polypeptide-like 1 preproprotein
• NCBI Official Synonym Full Names: prosaposin-like 1 (gene/pseudogene)
• NCBI Official Symbol: PSAPL1
• NCBI Protein Information: proactivator polypeptide-like 1; prosaposin-like protein 1
• UniProt Protein Name: Proactivator polypeptide-like 1
• Protein Family: Proactivator polypeptide
• UniProt Gene Name: PSAPL1
• UniProt Entry Name: SAPL1_HUMAN

NCBI Description

This gene encodes a protein that is related to the glycoprotein prosaposin. Based on sequence similarity between the encoded protein and prosaposin, it is predicted that the encoded protein is a preproprotein that is proteolytically processed to generate multiple protein products. These predicted products include saposins A-like, B-like, C-like, and D-like, which may play a role in the lysosomal degradation of sphingolipids. [provided by RefSeq, Jul 2015]

Uniprot Description

Function: May activate the lysosomal degradation of sphingolipids

By similarity.

Subcellular location: Secreted

Potential.

Sequence similarities: Contains 2 saposin A-type domains.Contains 4 saposin B-type domains.

Sequence caution: The sequence AAH68579.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC05143.1 differs from that shown. Reason: Erroneous termination at position 510. Translated as Trp.