Western Blot (WB)
(Western blot analysis of OLFML3 Antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane). OLFML3 (arrow) was detected using the purified Pab.)
Rabbit OLFML3 Polyclonal Antibody | anti-OLFML3 antibody
OLFML3 Antibody (Center)
Gene Names
OLFML3; OLF44; HNOEL-iso
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
OLFML3; Polyclonal Antibody; OLFML3 Antibody (Center); Olfactomedin-like protein 3; HNOEL-iso; hOLF44; anti-OLFML3 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This OLFML3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-134 amino acids from the Central region of human OLFML3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
107-134
Sequence Length
287
Applicable Applications for anti-OLFML3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Function
Secreted scaffold protein that plays an essential role in dorsoventral patterning during early development. Stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal side. Acts by facilitating the association between the tolloid proteases and their substrate chordin (CHRD), leading to enhance chordin (CHRD) degradation (By similarity). May have matrix-related function involved in placental and embryonic development, or play a similar role in other physiological processes.
Cellular Location
Secreted.
Tissue Location
Abundant in placenta, moderate in liver and heart, whereas fairly weak in other tissues examined. On term placenta, mainly localized extracellularly surrounding the syncytiotrophoblastic cells and very rarely expressed in the maternal decidua layer
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of OLFML3 Antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane). OLFML3 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of OLFML3 Antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane). OLFML3 (arrow) was detected using the purified Pab.)
Product Categories/Family for anti-OLFML3 antibody
References
Fautsch, M.P., et al. Exp. Eye Res. 82(6):1046-1052(2006)
Zeng, L.C., et al. FEBS Lett. 571 (1-3), 74-80 (2004)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
Zeng, L.C., et al. FEBS Lett. 571 (1-3), 74-80 (2004)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46010
NCBI Official Full Name
olfactomedin-like protein 3 isoform 1
NCBI Official Synonym Full Names
olfactomedin-like 3
NCBI Official Symbol
OLFML3
NCBI Official Synonym Symbols
OLF44; HNOEL-iso
NCBI Protein Information
olfactomedin-like protein 3
UniProt Protein Name
Olfactomedin-like protein 3
Protein Family
UniProt Gene Name
OLFML3
UniProt Entry Name
OLFL3_HUMAN
Uniprot Description
OLFML3: Secreted scaffold protein that plays an essential role in dorsoventral patterning during early development. Stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal side. Acts by facilitating the association between the tolloid proteases and their substrate chordin (CHRD), leading to enhance chordin (CHRD) degradation. May have matrix-related function involved in placental and embryonic development, or play a similar role in other physiological processes. Belongs to the OLFML3 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p13.2
Cellular Component: extracellular region; vesicle
Biological Process: multicellular organismal development
Research Articles on OLFML3
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Product Notes
The OLFML3 olfml3 (Catalog #AAA9204373) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 107-134. The OLFML3 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's OLFML3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the OLFML3 olfml3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OLFML3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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