Rabbit anti-Human PRSS36v Polyclonal Antibody | anti-PRSS36v antibody

PRSS36v (PRSS36, Polyserase-2, Polyserine protease 2, Serine protease 36) (Azide free) (HRP)

Cat. Num. AAA6337956

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRSS36v; Polyclonal Antibody; PRSS36v (PRSS36; Polyserase-2; Polyserine protease 2; Serine protease 36) (Azide free) (HRP); PRSS36; Serine protease 36; anti-PRSS36v antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PRSS36v antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PRSS36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-317 amino acids from the Central region of human PRSS36.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PRSS36 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PRSS36 antibody detected the PRSS36 protein (arrow).)

Related Product Information for anti-PRSS36v antibody

Serine protease. Hydrolyzes the peptides N-t-Boc-Gln-Ala-Arg-AMC and N-t-Boc-Gln-Gly-Arg-AMC and, to a lesser extent, N-t-Boc-Ala-Phe-Lys-AMC and N-t-Boc-Val-Leu-Lys-AMC. Has a preference for substrates with an Arg instead of a Lys residue in position P1.

Product Categories/Family for anti-PRSS36v antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005). Cal, S., et al. J. Biol. Chem. 280(3):1953-1961(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 58218970
• NCBI GeneID: 146547
• NCBI Accession #: NP_775773.2
• NCBI GenBank Nucleotide #: NM_173502.4
• UniProt Accession #: Q5K4E3; Q8NBY4; A8K2P5; B4DW80; B7ZMK8; E7EX56
• Molecular Weight: 91,422 Da
• NCBI Official Full Name: polyserase-2 isoform 1
• NCBI Official Synonym Full Names: protease, serine 36
• NCBI Official Symbol: PRSS36
• NCBI Protein Information: polyserase-2
• UniProt Protein Name: Polyserase-2
• UniProt Gene Name: PRSS36

Uniprot Description

PRSS36: Serine protease. Hydrolyzes the peptides N-t-Boc-Gln- Ala-Arg-AMC and N-t-Boc-Gln-Gly-Arg-AMC and, to a lesser extent, N-t-Boc-Ala-Phe-Lys-AMC and N-t-Boc-Val-Leu-Lys-AMC. Has a preference for substrates with an Arg instead of a Lys residue in position P1. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytoplasm; proteinaceous extracellular matrix

Molecular Function: serine-type endopeptidase activity

Biological Process: proteolysis