Rabbit anti-Human PRSS3 Polyclonal Antibody | anti-PRSS3 antibody

PRSS3, ID (PRSS3, PRSS4, TRY3, TRY4, Trypsin-3, Brain trypsinogen, Mesotrypsinogen, Serine protease 3, Serine protease 4, Trypsin III, Trypsin IV) (Biotin)

Cat. Num. AAA6337932

• Gene Names: PRSS3; T9; MTG; TRY3; TRY4; PRSS4
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRSS3; Polyclonal Antibody; ID (PRSS3; PRSS4; TRY3; TRY4; Trypsin-3; Brain trypsinogen; Mesotrypsinogen; Serine protease 3; Serine protease 4; Trypsin III; Trypsin IV) (Biotin); Trypsin IV; anti-PRSS3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PRSS3 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PRSS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-171 amino acids from the Central region of human PRSS3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(PRSS3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRSS3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(PRSS3 Antibody (Center) western blot analysis in HL-60,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PRSS3 antibody detected the PRSS3 protein (arrow).)

Flow Cytometry (FC/FACS)

(PRSS3 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PRSS3 antibody

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene.

Product Categories/Family for anti-PRSS3 antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Jiang, G., et al. Gut 59(11):1535-1544(2010). Nakanishi, J., et al. J. Invest. Dermatol. 130(4):944-952(2010). Salameh, M.A., et al. J. Biol. Chem. 285(3):1939-1949(2010). Rosendahl, J., et al. Pancreatology 10 (2-3), 243-249 (2010): Koistinen, H., et al. Neuroscience 160(1):97-102(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 21536452
• NCBI GeneID: 5646
• NCBI Accession #: NP_002762.2
• NCBI GenBank Nucleotide #: NM_002771.3
• UniProt Accession #: P35030; P15951; Q15665; Q5VXV0; Q9UQV3; A8CED1; A9Z1Y4; E7ES07
• Molecular Weight: 32,529 Da
• NCBI Official Full Name: trypsin-3 isoform 2 preproprotein
• NCBI Official Synonym Full Names: protease, serine, 3
• NCBI Official Symbol: PRSS3
• NCBI Official Synonym Symbols: T9; MTG; TRY3; TRY4; PRSS4
• NCBI Protein Information: trypsin-3; trypsin IV; mesotrypsin; trypsin III; trypsinogen 4; trypsinogen 5; trypsinogen IV; mesotrypsinogen; brain trypsinogen; pancreatic trypsinogen III; protease, serine, 4 (trypsin 4, brain)
• UniProt Protein Name: Trypsin-3
• UniProt Gene Name: PRSS3
• UniProt Synonym Gene Names: PRSS4; TRY3; TRY4
• UniProt Entry Name: TRY3_HUMAN

Uniprot Description

Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5.