Rat Transferrin Receptor (TFR) ELISA Kit | TFR elisa kit

Rat Transferrin Receptor (TFR) ELISA Kit

Cat. Num. AAA2702979

• Gene Names: TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
• Reactivity: Rat
• Synonyms: Transferrin Receptor (TFR); Rat Transferrin Receptor (TFR) ELISA Kit; CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1; serum form; TFR elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transferrin Receptor (TFR).; No significant cross-reactivity or interference between Transferrin Receptor (TFR) and analogues was observed.
• Sequence Length: 760

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.237ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transferrin Receptor (TFR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transferrin Receptor (TFR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TFR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transferrin Receptor (TFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transferrin Receptor (TFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transferrin Receptor (TFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transferrin Receptor (TFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 339516
• NCBI GeneID: 7037
• UniProt Accession #: P02786; Q1HE24; Q59G55; Q9UCN0; Q9UCU5; Q9UDF9; Q9UK21; D3DXB0
• Molecular Weight: 84,871 Da
• NCBI Official Full Name: transferrin receptor
• NCBI Official Synonym Full Names: transferrin receptor
• NCBI Official Symbol: TFRC
• NCBI Official Synonym Symbols: T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
• NCBI Protein Information: transferrin receptor protein 1
• UniProt Protein Name: Transferrin receptor protein 1
• UniProt Gene Name: TFRC
• UniProt Synonym Gene Names: TR; TfR; TfR1; Trfr; sTfR

NCBI Description

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Uniprot Description

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).