Rabbit anti-Human PROX1 Polyclonal Antibody | anti-PROX1 antibody

PROX1, CT (Prospero Homeobox Protein 1, Homeobox Prospero-like Protein PROX1, PROX-1) (Biotin)

Cat. Num. AAA6495920

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PROX1; Polyclonal Antibody; CT (Prospero Homeobox Protein 1; Homeobox Prospero-like Protein PROX1; PROX-1) (Biotin); anti-PROX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PROX1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PROX1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:50-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region (between a portion within aa500~529) of human PROX1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PROX1 (arrow) using rabbit polyclonal PROX1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PROX1 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(PROX1 Antibody (K507) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PROX1 antibody detected the PROX1 protein (arrow).)

Immunofluorescence (IF)

(Fluorescent confocal image of A2058 cell stained with PROX1 Antibody (C-term).A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PROX1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). PROX1 immunoreactivity is localized to Nucleus significantly.)

Product Categories/Family for anti-PROX1 antibody

Antibodies; Transcription Factors; Homeobox (HOX)

References

1. Nagai, H., et al., Genes Chromosomes Cancer 38(1):13-21 (2003). 2. Dyer, M.A., et al., Nat. Genet. 34(1):53-58 (2003). 3. Hong, Y.K., et al., Dev. Dyn. 225(3):351-357 (2002). 4. Petrova, T.V., et al., EMBO J. 21(17):4593-4599 (2002). 5. Mouta Carreira, C., et al., Cancer Res. 61(22):8079-8084 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 21359846
• NCBI GeneID: 5629
• NCBI Accession #: NP_002754
• NCBI GenBank Nucleotide #: NM_002763.4
• UniProt Accession #: Q92786
• Molecular Weight: 81kDa
• NCBI Official Full Name: prospero homeobox protein 1
• NCBI Official Synonym Full Names: prospero homeobox 1
• NCBI Official Symbol: PROX1
• NCBI Protein Information: prospero homeobox protein 1
• UniProt Protein Name: Prospero homeobox protein 1
• Protein Family: Prospero homeobox protein
• UniProt Gene Name: PROX1
• UniProt Synonym Gene Names: PROX-1
• UniProt Entry Name: PROX1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

Uniprot Description

PROX1: May play a fundamental role in early development of CNS. May regulate gene expression and development of postmitotic undifferentiated young neurons. Belongs to the Prospero homeobox family.

Protein type: DNA-binding; Transcription factor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor binding; protein binding; DBD domain binding; DNA binding; LBD domain binding; transcription factor activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; lens development in camera-type eye; circadian rhythm; neural tube development; positive regulation of cyclin-dependent protein kinase activity; ventricular cardiac myofibril development; muscle thin filament assembly; atrial cardiac muscle morphogenesis; positive regulation of transcription, DNA-dependent; cell fate determination; negative regulation of transcription from RNA polymerase II promoter; olfactory placode formation; negative regulation of cell proliferation; venous blood vessel morphogenesis; pancreas development; positive regulation of cell proliferation; lymphangiogenesis; ventricular cardiac muscle morphogenesis; kidney development; response to nutrient levels; dentate gyrus development; negative regulation of transcription factor activity; positive regulation of cell cycle; liver development; regulation of circadian rhythm; cerebellar granule cell differentiation; dorsal spinal cord development; negative regulation of viral genome replication; regulation of gene expression; otic placode formation; embryonic retina morphogenesis in camera-type eye; positive regulation of endothelial cell proliferation; optic placode formation involved in camera-type eye; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; retina morphogenesis in camera-type eye; lung development