Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse PTPRC Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Protein Tyrosine Phosphatase Receptor Type C (PTPRC) Polyclonal Antibody | anti-PTPRC antibody
Polyclonal Antibody to Protein Tyrosine Phosphatase Receptor Type C (PTPRC)
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- FQSIPRV FSKFPIKDAR KPHNQNKNRY VDILPYDYNR VELSEINGDA GSTYINASYI DGFKEPRKYI AAQGPRDETV DDFWRMIWEQ KATVIVMVTR CEEGNRNKCA EYWPSMEEGT RAFKDIVVTI NDHKRCPDYI IQKLNVAHKK EKATGREVTH IQFTSWPDHG VPEDPHLLLK LRRRVNAFSN FFSGPIVVHC SAGVGRTGTY IGIDAMLEGL EAEGKVDVYG YVVKLRRQRC LMVQVEAQYI LIHQALVEYN
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse PTPRC Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse PTPRC Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
NCBI and Uniprot Product Information
Uniprot Description
CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.
Protein type: EC 3.1.3.48; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine
Chromosomal Location of Human Ortholog: 1 E4|1 60.73 cM
Cellular Component: cell periphery; cell surface; external side of plasma membrane; extracellular exosome; focal adhesion; integral component of membrane; integral component of plasma membrane; membrane; plasma membrane
Molecular Function: heparan sulfate proteoglycan binding; heparin binding; hydrolase activity; phosphoprotein phosphatase activity; phosphoric monoester hydrolase activity; protein binding; protein kinase binding; protein tyrosine phosphatase activity
Biological Process: activation of MAPK activity; B cell differentiation; B cell proliferation; B cell receptor signaling pathway; bone marrow development; cell cycle phase transition; defense response to virus; dephosphorylation; hemopoietic progenitor cell differentiation; heterotypic cell-cell adhesion; immunoglobulin biosynthetic process; leukocyte adhesion; negative regulation of cell adhesion involved in substrate-bound cell migration; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of protein autophosphorylation; negative regulation of protein kinase activity; negative regulation of T cell mediated cytotoxicity; negative thymic T cell selection; positive regulation of alpha-beta T cell proliferation; positive regulation of antigen receptor-mediated signaling pathway; positive regulation of B cell proliferation; positive regulation of extrinsic apoptotic signaling pathway; positive regulation of gamma-delta T cell differentiation; positive regulation of hematopoietic stem cell migration; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of isotype switching to IgG isotypes; positive regulation of MAPK cascade; positive regulation of stem cell proliferation; positive regulation of T cell differentiation; positive regulation of T cell mediated cytotoxicity; positive regulation of T cell mediated immunity; positive regulation of T cell proliferation; positive thymic T cell selection; protein amino acid dephosphorylation; regulation of B cell differentiation; regulation of B cell receptor signaling pathway; regulation of cell cycle; regulation of extrinsic apoptotic signaling pathway; regulation of humoral immune response mediated by circulating immunoglobulin; release of sequestered calcium ion into cytosol; response to gamma radiation; stem cell development; T cell differentiation; T cell proliferation; T cell receptor signaling pathway
Research Articles on PTPRC
Similar Products
Product Notes
The PTPRC ptprc (Catalog #AAA2005067) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Protein Tyrosine Phosphatase Receptor Type C (PTPRC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Tyrosine Phosphatase Receptor Type C (PTPRC) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PTPRC ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- FQSIPRV FSKFPIKDAR KPHNQNKNRY VDILPYDYNR VELSEINGDA GSTYINASYI DGFKEPRKYI AAQGPRDETV DDFWRMIWEQ KATVIVMVTR CEEGNRNKCA EYWPSMEEGT RAFKDIVVTI NDHKRCPDYI IQKLNVAHKK EKATGREVTH IQFTSWPDHG VPEDPHLLLK LRRRVNAFSN FFSGPIVVHC SAGVGRTGTY IGIDAMLEGL EAEGKVDVYG YVVKLRRQRC LMVQVEAQYI LIHQALVEYN. It is sometimes possible for the material contained within the vial of "Protein Tyrosine Phosphatase Receptor Type C (PTPRC), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
