Rabbit anti-Mouse Protein Kinase R Polyclonal Antibody | anti-PKR antibody

Polyclonal Antibody to Protein Kinase R (PKR)

Cat. Num. AAA2026865

• Gene Names: Eif2ak2; Pkr; Tik; Prkr; AI467567; AI747578; 2310047A08Rik; 4732414G15Rik
• Reactivity: Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Protein Kinase R; Polyclonal Antibody; Polyclonal Antibody to Protein Kinase R (PKR); anti-PKR antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against PKR. It has beenselected for its ability to recognize PKR in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid
• Concentration: 500ug/mL (varies by lot)

• Applicable Applications for anti-PKR antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 0.2-2ug/mL;1:250-2500; Immunohistochemistry: 5-20ug/mL;1:25-100; Immunocytochemistry: 5-20ug/mL;1:25-100; Optimal working dilutions must be determined by end user.
• Fragment: PKR (Asn205~Glu495)
• Organism Species: Mus musculus (Mouse)
• Cross Reactivity: Mouse
• Conjugate: No Conjugate
• Immunogen: Recombinant PKR (Asn205~Glu495) expressed in E Coli.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2043312
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded stomach tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Kidney Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 6755160
• NCBI GeneID: 19106
• NCBI Accession #: NP_035293.1
• NCBI GenBank Nucleotide #: NM_011163.4
• UniProt Accession #: Q03963; Q61742; Q62026
• Molecular Weight: 58,280 Da
• NCBI Official Full Name: interferon-induced, double-stranded RNA-activated protein kinase
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 2
• NCBI Official Symbol: Eif2ak2
• NCBI Official Synonym Symbols: Pkr; Tik; Prkr; AI467567; AI747578; 2310047A08Rik; 4732414G15Rik
• NCBI Protein Information: interferon-induced, double-stranded RNA-activated protein kinase
• UniProt Protein Name: Interferon-induced, double-stranded RNA-activated protein kinase
• Protein Family: Protein kinase
• UniProt Gene Name: Eif2ak2
• UniProt Synonym Gene Names: Pkr; Prkr; Tik; eIF-2A protein kinase 2; PKR; Protein kinase R

Uniprot Description

PKR: a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation.

Protein type: EC 2.7.10.2; EC 2.7.11.1; Kinase, protein; Other group; PEK family; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Translation

Chromosomal Location of Human Ortholog: 17 E3|17 49.56 cM

Cellular Component: cytoplasm; cytosol; membrane; nucleus; perinuclear region of cytoplasm

Molecular Function: ATP binding; double-stranded RNA binding; eukaryotic translation initiation factor 2alpha kinase activity; identical protein binding; kinase activity; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein tyrosine kinase activity; RNA binding; transferase activity

Biological Process: activation of MAPKK activity; cellular response to amino acid starvation; defense response to virus; endoplasmic reticulum unfolded protein response; immune system process; innate immune response; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of osteoblast proliferation; negative regulation of translation; negative regulation of viral genome replication; phosphorylation; positive regulation of apoptosis; positive regulation of chemokine production; positive regulation of cytokine production; positive regulation of NF-kappaB transcription factor activity; positive regulation of NIK/NF-kappaB signaling; positive regulation of stress-activated MAPK cascade; protein amino acid phosphorylation; protein autophosphorylation; regulation of apoptosis; regulation of hematopoietic progenitor cell differentiation; regulation of hematopoietic stem cell differentiation; regulation of hematopoietic stem cell proliferation; regulation of NLRP3 inflammasome complex assembly; regulation of transcription, DNA-templated; regulation of translational initiation by eIF2 alpha phosphorylation; response to lipopolysaccharide; response to toxin; response to virus; response to vitamin E; transcription, DNA-dependent; translation