Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Protein Kinase C Zeta (PKCz) Polyclonal Antibody | anti-PKCz antibody
Polyclonal Antibody to Protein Kinase C Zeta (PKCz)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LIRVIGR GSYAKVLLVR LKKNDQIYAM KVVKKELVHD DEDIDWVQTE KHVFEQASSN PFLVGLHSCF QTTSRLFLVI EYVNGGDLMF HMQRQRKLPE EHARFYAAEI CIALNFLHER GIIYRDLKLD NVLLDADGHI KLTDYGMCKE GLGPGDTTST FCGTPNYIAP EILRGEEYGF SVDWWALGVL MFEMMAGRSP
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
NCBI and Uniprot Product Information
Uniprot Description
PKCZ: an AGC kinase of the PKC family. An atypical PKC: its activity is not regulated by Ca2+, PS, DAG or phorbol esters. Constitutively active.
Protein type: AGC group; EC 2.7.11.13; Iota subfamily; Kinase, protein; PKC family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 4 E2|4 86.17 cM
Cellular Component: apical cortex; apical plasma membrane; axon hillock; bicellular tight junction; cell cortex; cell junction; cell leading edge; cytoplasm; cytosol; endosome; extracellular exosome; filamentous actin; intercellular junction; intracellular membrane-bound organelle; membrane raft; microtubule organizing center; nuclear envelope; nuclear matrix; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; vesicle
Molecular Function: 14-3-3 protein binding; ATP binding; kinase activity; metal ion binding; nucleotide binding; phospholipase binding; potassium channel regulator activity; protein binding; protein domain specific binding; protein kinase activity; protein kinase binding; protein kinase C activity; protein serine/threonine kinase activity; transferase activity
Biological Process: actin cytoskeleton reorganization; activation of protein kinase B activity; cell migration; cell surface receptor signaling pathway; cellular protein localization; cellular response to insulin stimulus; establishment of cell polarity; inflammatory response; insulin receptor signaling pathway; long-term memory; membrane depolarization; membrane hyperpolarization; microtubule cytoskeleton organization; negative regulation of apoptosis; negative regulation of hydrolase activity; negative regulation of insulin receptor signaling pathway; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of protein complex assembly; peptidyl-serine phosphorylation; phospholipase D activation; phosphorylation; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of ERK1 and ERK2 cascade; positive regulation of excitatory postsynaptic potential; positive regulation of glucose import; positive regulation of insulin receptor signaling pathway; positive regulation of interleukin-10 secretion; positive regulation of interleukin-13 secretion; positive regulation of interleukin-4 production; positive regulation of interleukin-5 secretion; positive regulation of NF-kappaB transcription factor activity; positive regulation of protein transport; positive regulation of synaptic transmission; positive regulation of T-helper 2 cell cytokine production; positive regulation of T-helper 2 cell differentiation; protein amino acid phosphorylation; protein heterooligomerization; protein localization to plasma membrane; signal transduction; vesicle transport along microtubule
Research Articles on PKCz
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Product Notes
The PKCz prkcz (Catalog #AAA2016880) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Protein Kinase C Zeta (PKCz) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Kinase C Zeta (PKCz) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PKCz prkcz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LIRVIGR GSYAKVLLVR LKKNDQIYAM KVVKKELVHD DEDIDWVQTE KHVFEQASSN PFLVGLHSCF QTTSRLFLVI EYVNGGDLMF HMQRQRKLPE EHARFYAAEI CIALNFLHER GIIYRDLKLD NVLLDADGHI KLTDYGMCKE GLGPGDTTST FCGTPNYIAP EILRGEEYGF SVDWWALGVL MFEMMAGRSP. It is sometimes possible for the material contained within the vial of "Protein Kinase C Zeta (PKCz), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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