Mouse anti-Human, Mouse RELB Monoclonal Antibody | anti-RELB antibody

RELB Antibody

Cat. Num. AAA9217058

• Gene Names: RELB; IREL; I-REL; REL-B
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Synonyms: RELB; Monoclonal Antibody; RELB Antibody; Transcription factor RelB; I-Rel; anti-RELB antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Specificity: This RELB antibody is generated from a mouse immunized with a recombinant protein human RELB.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 579

• Applicable Applications for anti-RELB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:5000
• Subtitle: Purified Mouse Monoclonal Antibody (Mab)
• Cellular Location: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with NEK6 in the centrosome.
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-RELB Antibody at 1:5000 dilution + Daudi whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-RELB Antibody at 1:5000 dilutionLane 1: Raji whole cell lysateLane 2: Jurkat whole cell lysateLane 3: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RELB antibody

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF- kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF- kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a CRY1/CRY2 independent manner. Increased repression of the heterodimer is seen in the presence of NFKB2/p52.

Product Categories/Family for anti-RELB antibody

Crown Antibodies; Primary Antibodies; Cancer; Cell Biology; Signal Transduction

References

Ruben S.M.,et al.Genes Dev. 6:745-760 (1992). Yoshiura K.,et al.Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases. Ryseck R.P.,et al.Mol. Cell. Biol. 12:674-684 (1992). Dobrzanski P.,et al.Mol. Cell. Biol. 13:1572-1582 (1993). Dobrzanski P.,et al.EMBO J. 13:4608-4616 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 35493878
• NCBI GeneID: 5971
• NCBI Accession #: NP_006500.2
• NCBI GenBank Nucleotide #: NM_006509.3
• UniProt Accession #: Q01201; Q6GTX7; Q9UEI7
• Molecular Weight: 62134 Da
• NCBI Official Full Name: transcription factor RelB
• NCBI Official Synonym Full Names: RELB proto-oncogene, NF-kB subunit
• NCBI Official Symbol: RELB
• NCBI Official Synonym Symbols: IREL; I-REL; REL-B
• NCBI Protein Information: transcription factor RelB
• UniProt Protein Name: Transcription factor RelB
• Protein Family: Antitoxin
• UniProt Gene Name: RELB
• UniProt Entry Name: RELB_HUMAN

Uniprot Description

RELB: a transcription factor of the nuclear factor-kappaB (NFkB) transcription complex. Stimulates promoter activity in the presence NFkB1/NFkB-p50. Plays role in regulation of apoptosis in hepatocellular carcinoma through activation of downstream target genes. Does not associate with DNA or RelA/NFkB-p65.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytoplasm; cytosol; microtubule organizing center; nucleoplasm; nucleus

Molecular Function: chromatin binding; protein binding; transcription corepressor activity; transcription factor activity

Biological Process: antigen processing and presentation; circadian regulation of gene expression; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; myeloid dendritic cell differentiation; negative regulation of interferon-beta production; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; response to cytokine stimulus; stimulatory C-type lectin receptor signaling pathway; T-helper 1 cell differentiation; transcription, DNA-dependent