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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse Protein Kinase C Delta Polyclonal Antibody | anti-PKCd antibody

Polyclonal Antibody to Protein Kinase C Delta (PKCd)

Gene Names
Prkcd; Pkcd; PKC[d]; AI385711; PKCdelta; D14Ertd420e
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Protein Kinase C Delta; Polyclonal Antibody; Polyclonal Antibody to Protein Kinase C Delta (PKCd); anti-PKCd antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PKCd. It has beenselected for its ability to recognize PKCd in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
473
Applicable Applications for anti-PKCd antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
PKCd (Asn328~Ile674)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant PKCd (Asn328~Ile674) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2042334
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded spermaduct tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded spermaduct tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Stomach Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Stomach Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
80,294 Da
NCBI Official Full Name
protein kinase C delta type isoform 2
NCBI Official Synonym Full Names
protein kinase C, delta
NCBI Official Symbol
Prkcd
NCBI Official Synonym Symbols
Pkcd; PKC[d]; AI385711; PKCdelta; D14Ertd420e
NCBI Protein Information
protein kinase C delta type
UniProt Protein Name
Protein kinase C delta type
Protein Family
UniProt Gene Name
Prkcd
UniProt Synonym Gene Names
Pkcd; SDK1

Uniprot Description

PKCD: an AGC kinase of the PKC family. A novel PKC: Ca2 independent but still regulated by PS, DAG and phorbol esters. Caspase-3 appears to liberate a sphingosine-activated catalytic fragment that has altered regulatory mechanisms and substrate specificities.

Protein type: AGC group; Delta subfamily; EC 2.7.11.13; Kinase, protein; Nuclear receptor co-regulator; PKC family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor

Chromosomal Location of Human Ortholog: 14 B|14 18.82 cM

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; extracellular exosome; intercellular junction; membrane; mitochondrion; nuclear matrix; nucleus; plasma membrane

Molecular Function: ATP binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; insulin receptor substrate binding; kinase activity; kinase binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein kinase activity; protein kinase binding; protein kinase C activity; protein serine/threonine kinase activity; TIR domain binding; transferase activity

Biological Process: activation of protein kinase activity; apoptosis; aspartate transport; B cell proliferation; cell cycle; cellular response to angiotensin; cellular response to hydrogen peroxide; cellular response to hydroperoxide; cellular response to oxidative stress; cellular senescence; collagen metabolic process; defense response to bacterium; histone phosphorylation; immunoglobulin mediated immune response; interleukin-10 production; interleukin-12 production; negative regulation of actin filament polymerization; negative regulation of filopodium formation; negative regulation of insulin receptor signaling pathway; negative regulation of MAP kinase activity; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of platelet aggregation; neutrophil activation; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of apoptosis; positive regulation of apoptotic signaling pathway; positive regulation of ceramide biosynthetic process; positive regulation of endodeoxyribonuclease activity; positive regulation of glucose import; positive regulation of glucosylceramide catabolic process; positive regulation of MAP kinase activity; positive regulation of MAPK cascade; positive regulation of phospholipid scramblase activity; positive regulation of protein dephosphorylation; positive regulation of protein import into nucleus; positive regulation of response to DNA damage stimulus; positive regulation of sphingomyelin catabolic process; positive regulation of superoxide anion generation; protein amino acid phosphorylation; protein autophosphorylation; regulation of actin cytoskeleton organization; regulation of phosphorylation; response to oxidative stress

Research Articles on PKCd

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Product Notes

The PKCd prkcd (Catalog #AAA2027175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Protein Kinase C Delta (PKCd) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Kinase C Delta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the PKCd prkcd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein Kinase C Delta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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