Western Blot (WB)
(GPNMB Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the GPNMB antibody detected the GPNMB protein (arrow).)
Rabbit anti-Human GPNMB Polyclonal Antibody | anti-GPNMB antibody
GPNMB, CT (GPNMB, HGFIN, NMB, Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN) (PE)
Gene Names
GPNMB; NMB; HGFIN
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
GPNMB; Polyclonal Antibody; CT (GPNMB; HGFIN; NMB; Transmembrane glycoprotein NMB; Transmembrane glycoprotein HGFIN) (PE); Transmembrane glycoprotein HGFIN; anti-GPNMB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-GPNMB antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
GPNMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from the C-terminal region of human GPNMB.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(GPNMB Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the GPNMB antibody detected the GPNMB protein (arrow).)
Western Blot (WB)
(GPNMB Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the GPNMB antibody detected the GPNMB protein (arrow).)
Related Product Information for anti-GPNMB antibody
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-GPNMB antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Williams, M.D., et al. Melanoma Res. 20(3):184-190(2010). Hoashi, T., et al. FASEB J. 24(5):1616-1629(2010). Rose, A.A., et al. Clin. Cancer Res. 16(7):2147-2156(2010). Rose, A.A., et al. PLoS ONE 5 (8), E12093 (2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,643 Da
NCBI Official Full Name
transmembrane glycoprotein NMB isoform b
NCBI Official Synonym Full Names
glycoprotein (transmembrane) nmb
NCBI Official Symbol
GPNMB
NCBI Official Synonym Symbols
NMB; HGFIN
NCBI Protein Information
transmembrane glycoprotein NMB; osteoactivin; glycoprotein nmb-like protein; transmembrane glycoprotein HGFIN
UniProt Protein Name
Transmembrane glycoprotein NMB
Protein Family
UniProt Gene Name
GPNMB
UniProt Synonym Gene Names
HGFIN; NMB
UniProt Entry Name
GPNMB_HUMAN
NCBI Description
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
GPNMB: Could be a melanogenic enzyme. Belongs to the PMEL/NMB family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 7p15
Cellular Component: integral to plasma membrane; integral to membrane; melanosome
Molecular Function: heparin binding; integrin binding
Biological Process: osteoblast differentiation; negative regulation of cell proliferation; cell adhesion; bone mineralization
Research Articles on GPNMB
Similar Products
Product Notes
The GPNMB gpnmb (Catalog #AAA6303644) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPNMB, CT (GPNMB, HGFIN, NMB, Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPNMB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GPNMB gpnmb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPNMB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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