Rabbit anti-Mouse Prkcd Polyclonal Antibody | anti-Prkcd antibody

Prkcd, NT (Prkcd, Pkcd, Protein kinase C delta type, Tyrosine-protein kinase PRKCD, nPKC-delta) (Biotin)

Cat. Num. AAA6337327

• Gene Names: Prkcd; Pkcd; PKC[d]; AI385711; PKCdelta; D14Ertd420e
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Prkcd; Polyclonal Antibody; NT (Prkcd; Pkcd; Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta) (Biotin); nPKC-delta; anti-Prkcd antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-Prkcd antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Prkcd antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-67 amino acids from the N-terminal region of Mouse Prkcd.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Prkcd Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the Prkcd antibody detected the Prkcd protein (arrow).)

Related Product Information for anti-Prkcd antibody

This is calcium-independent, phospholipid-dependent, serine-and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin (By similarity).

Product Categories/Family for anti-Prkcd antibody

Antibodies; Protein Kinases

References

Ronda, A.C., et al. J. Steroid Biochem. Mol. Biol. 122(4):287-294(2010). Li, X., et al. J. Am. Soc. Nephrol. 21(7):1115-1124(2010). Romanova, L.Y., et al. J. Cell. Sci. 123 (PT 9), 1567-1577 (2010): White, M.C., et al. PLoS Genet. 6 (6), E1000984 (2010): Niger, C., et al. BMC Biochem. 11, 14 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 6755082
• NCBI GeneID: 18753
• NCBI Accession #: NP_035233.1
• NCBI GenBank Nucleotide #: NM_011103.3
• UniProt Accession #: P28867; Q91V85; Q9Z333
• Molecular Weight: 80,294 Da
• NCBI Official Full Name: protein kinase C delta type
• NCBI Official Synonym Full Names: protein kinase C, delta
• NCBI Official Symbol: Prkcd
• NCBI Official Synonym Symbols: Pkcd; PKC[d]; AI385711; PKCdelta; D14Ertd420e
• NCBI Protein Information: protein kinase C delta type; nPKC-delta; protein kinase C delta variant IX; protein kinase C, delta IV; protein kinase C, delta V; protein kinase C[d]; tyrosine-protein kinase PRKCD
• UniProt Protein Name: Protein kinase C delta type
• Protein Family: Protein kinase
• UniProt Gene Name: Prkcd
• UniProt Synonym Gene Names: Pkcd; SDK1

Uniprot Description

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. Negatively regulates B cell proliferation and also has an important function in self-antigen induced B cell tolerance induction. Upon DNA damage, activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. In response to oxidative stress, interact with and activate CHUK/IKKA in the nucleus, causing the phosphorylation of p53/TP53. In the case of ER stress or DNA damage-induced apoptosis, can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting AKT1 and decreasing the level of X-linked inhibitor of apoptosis protein (XIAP), whereas in nucleus induces apoptosis via the activation of MAPK8 or MAPK9. Upon ionizing radiation treatment, is required for the activation of the apoptosis regulators BAX and BAK, which trigger the mitochondrial cell death pathway. Can phosphorylate MCL1 and target it for degradation which is sufficient to trigger for BAX activation and apoptosis. Is required for the control of cell cycle progression both at G1/S and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-induced inhibition of cell cycle progression at G1/S phase by up-regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin CCNA2 promoter activity. In response to UV irradiation can phosphorylate CDK1, which is important for the G2/M DNA damage checkpoint activation. Can protect glioma cells from the apoptosis induced by TNFSF10/TRAIL, probably by inducing increased phosphorylation and subsequent activation of AKT1. Can also act as tumor suppressor upon mitogenic stimulation with PMA or TPA. In N-formyl-methionyl-leucyl-phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-phox) phosphorylation and activation of NADPH oxidase activity, and regulates TNF-elicited superoxide anion production in neutrophils, by direct phosphorylation and activation of NCF1 or indirectly through MAPK1/3 (ERK1/2) signaling pathways. May also play a role in the regulation of NADPH oxidase activity in eosinophil after stimulation with IL5, leukotriene B4 or PMA. In collagen-induced platelet aggregation, acts a negative regulator of filopodia formation and actin polymerization by interacting with and negatively regulating VASP phosphorylation. Downstream of PAR1, PAR4 and CD36/GP4 receptors, regulates differentially platelet dense granule secretion; acts as a positive regulator in PAR-mediated granule secretion, whereas it negatively regulates CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. The catalytic subunit phosphorylates 14-3-3 proteins (YWHAB, YWHAZ and YWHAH) in a sphingosine-dependent fashion. Phosphorylates ELAVL1 in response to angiotensin-2 treatment ().