Goat anti-Human, Rat Protein Kinase C beta1 Polyclonal Antibody | anti-Prkcb antibody

Protein Kinase C beta1 (PKCb1)

Cat. Num. AAA613159

• Gene Names: Prkcb; Pkcb; Prkcb1
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by human PKCb1 affinity chromatography.
• Synonyms: Protein Kinase C beta1; Polyclonal Antibody; Protein Kinase C beta1 (PKCb1); Anti -Protein Kinase C beta1 (PKCb1); anti-Prkcb antibody

• Host: Goat
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.
• Purity/Purification: Affinity Purified; Purified by human PKCb1 affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

• Applicable Applications for anti-Prkcb antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect PKC 1. The detection limit for rhPKC 1 is approximately 0.5 ng/lane under non-reducing and reducing conditions., Immunocytochemistry: This antibody has been used at a concentration of 10ug/ml to detect PKC 1 in neural progenitor cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4 degree C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human PKC 1. The detection limit for rhPKC 1 is approximately 0.3 ng/well. In this format, this antibody shows less than 1% cross-reactivity with rhPKC., Optimal dilutions should be determined by each laboratory for each application.
• Immunogen: E. coli-derived rhPKCb1 (aa607-671)
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Prkcb antibody

PKC 1 is a 671 aa, 77kD member of the PKC subfamily of Ser/Thr family of protein kinases. PKC 1 is a Ca2+ -activated, phospholipid-dependent enzyme responsible for the phosphorylation of multiple proteins. Human PKC 2 is an alternatively spliced isoform with a 52 aa substitution of the 50 C-terminal residues. In monkey, five alternativly spliced forms have been reported. Over the region used as immunogen, human and rat PKC 1 are 100% identical.

Product Categories/Family for anti-Prkcb antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 288806594
• NCBI GeneID: 25023
• NCBI Accession #: NP_001165776.1
• NCBI GenBank Nucleotide #: NM_001172305.1
• UniProt Accession #: P68403; P04410; P04411
• Molecular Weight: 76,751 Da
• NCBI Official Full Name: protein kinase C beta type isoform 2
• NCBI Official Synonym Full Names: protein kinase C, beta
• NCBI Official Symbol: Prkcb
• NCBI Official Synonym Symbols: Pkcb; Prkcb1
• NCBI Protein Information: protein kinase C beta type; PKC-beta; protein kinase C beta I; protein kinase C beta II; protein kinase C, beta 1
• UniProt Protein Name: Protein kinase C beta type
• Protein Family: Protein kinase
• UniProt Gene Name: Prkcb
• UniProt Synonym Gene Names: Pkcb; Prkcb1; PKC-B; PKC-beta
• UniProt Entry Name: KPCB_RAT

NCBI Description

plays a role in hexosamine biosynthesis pathway induced transcriptional regulation [RGD, Feb 2006]

Uniprot Description

PKCB: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic receptors. Activates the NF-kappaB signaling pathway in B cells after antigen-receptor signaling. Two splice-variant isoforms have been described.

Protein type: Kinase, protein; EC 2.7.11.13; Motility/polarity/chemotaxis; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Alpha subfamily

Cellular Component: centrosome; membrane; brush border membrane; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; protein kinase C binding; zinc ion binding; histone binding; calcium channel regulator activity; chromatin binding; ATP binding

Biological Process: response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; B cell activation; transcription, DNA-dependent; dibenzo-p-dioxin metabolic process; apoptosis; positive regulation of B cell receptor signaling pathway; negative regulation of insulin receptor signaling pathway; positive regulation of odontogenesis of dentine-containing teeth; regulation of dopamine secretion; protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; regulation of transcription from RNA polymerase II promoter; positive regulation of angiogenesis; B cell receptor signaling pathway; response to vitamin D; response to ethanol; regulation of growth; calcium ion transport; response to glucose stimulus; response to hypoxia