Rabbit anti-Human, Mouse Protein Kinase C, alpha, beta2 Polyclonal Antibody | anti-Prkcb antibody

Protein Kinase C, alpha, beta2 (Thr638/641) (PKCalpha/beta2)

Cat. Num. AAA619426

• Gene Names: Prkcb; Pkcb; Prkcb1; Prkcb2; PKC-Beta; A130082F03Rik
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Protein Kinase C; alpha; beta2; Polyclonal Antibody; beta2 (Thr638/641) (PKCalpha/beta2); Anti -Protein Kinase C; beta2 (Thr638/641) (PKC / 2); anti-Prkcb antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of total human and mouse PKC and PKC 2 protein.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, pH 7.4, 0.02% sodium azide.

• Applicable Applications for anti-Prkcb antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot; Dilution: ELISA: 0.05-0.2ug/ml; Western Blot: 0.5-2ug/ml
• Immunogen: Synthetic non-phosphopeptide (KLH). corresponding to residues surrounding Thr638 of human PKCalpha and Thr641 of human PKCbeta2.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of lysates from TPA-treated Hela cells using Rabbit Anti-PKCα/β2 (Thr638/641) Polyclonal Antibody . Lane 1: Rabbit Anti-PKCα/β2 (Phospho-Thr638/641) Polyclonal Antibody Lane 2: Rabbit Anti-PKCα/β2 (Phospho-Thr638/641) Polyclonal Antibody pre-incubated with phospho-peptide Lane 3: Rabbit Anti-PKCα/β2 (Phospho-Thr638/641) Polyclonal Antibody pre-incubated with non-phospho-peptide Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody The signal was developed with LumiSensorTM HRP Substrate Kit)

Western Blot (WB)

(Western blot analysis of NIH/3T3 cell lysate using Rabbit Anti-PKCα/β2 (Thr638/641) Polyclonal Antibody .)

Related Product Information for anti-Prkcb antibody

Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. The PKC family has been divided into three groups by the enzymes' cofactor requirements: conventional (c)PKC isoforms (comprising a, bI (also known as b1), bII (also known as b2) and g), that require diacylglycerol (DAG), Ca2+, and phospholipid for activation; novel (n)PKC isoforms (comprising d, e, eta (also known as PKC-L), Phi and µ (the mouse homolog of human PKCµ, known as PKD)) that require DAG but not Ca2+; and atypical (a)PKC isoforms, namely zeta, iota, and lambda (the mouse homolog of human PKC) that require neither Ca2+ nor DAG. A new PKC member has recently been discovered and is referred to as PKC.

Product Categories/Family for anti-Prkcb antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 6679345
• NCBI GeneID: 18751
• NCBI Accession #: NP_032881.1
• NCBI GenBank Nucleotide #: NM_008855.2
• UniProt Accession #: P68404; P04410; P04411; A0JNZ5; F2Z441
• Molecular Weight: 76,751 Da
• NCBI Official Full Name: protein kinase C beta type
• NCBI Official Synonym Full Names: protein kinase C, beta
• NCBI Official Symbol: Prkcb
• NCBI Official Synonym Symbols: Pkcb; Prkcb1; Prkcb2; PKC-Beta; A130082F03Rik
• NCBI Protein Information: protein kinase C beta type; PKC-B; protein kinase C beta-II; protein kinase C, beta 1
• UniProt Protein Name: Protein kinase C beta type
• Protein Family: Protein kinase
• UniProt Gene Name: Prkcb
• UniProt Synonym Gene Names: Pkcb; Prkcb1; PKC-B; PKC-beta
• UniProt Entry Name: KPCB_MOUSE

Uniprot Description

PKCB iso2: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic receptors. Activates the NF-kappaB signaling pathway in B cells after antigen-receptor signaling. Two splice-variant isoforms have been described.

Protein type: EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; Motility/polarity/chemotaxis; AGC group; PKC family; Alpha subfamily

Cellular Component: centrosome; membrane; brush border membrane; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein kinase C activity; zinc ion binding; histone binding; metal ion binding; calcium channel regulator activity; nucleotide binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; protein kinase C binding; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; transferase activity, transferring phosphorus-containing groups; chromatin binding; kinase activity; ATP binding

Biological Process: response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; B cell activation; transcription, DNA-dependent; apoptosis; immune system process; positive regulation of B cell receptor signaling pathway; negative regulation of insulin receptor signaling pathway; chromatin modification; regulation of dopamine secretion; protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; regulation of transcription from RNA polymerase II promoter; B cell receptor signaling pathway; positive regulation of angiogenesis; regulation of transcription, DNA-dependent; regulation of growth; calcium ion transport; response to hypoxia; phosphorylation