Protein A Secondary Antibody | faeC secondary antibody
Protein A-UNLB
Applications
ELISA, Immunoblot, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
Protein A; Polyclonal Secondary Antibody; Protein A-UNLB; Protein A - Purified; Unlabeled Antibody; faeC secondary antibody
Host
Staphylococcus aureus
Clonality
Polyclonal
Specificity
Protein A binds the Fc portion of human IgG1, IgG2, IgG3 (weakly), IgG4, and mouse IgG1 (weakly), IgG2a, IgG2b, and IgG3. Protein A also binds IgG from monkey, rabbit, pig, guinea pig, dog, and cat.
Form/Format
Purified (UNLB) Protein A
Sequence Length
181
Applicable Applications for faeC secondary antibody
ELISA; Immunoblot; Immunohistochemistry; Immunoprecipitation
Mr/Structure
42-kDa. Protein A is a monomeric protein lacking cysteine residues.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by FPLC and ELISA to conform to the characteristics of a standard reference reagent. Protein A-HRP is prepared using highly purified horseradish peroxidase (RZ > 3.0).
Preparation and Storage
The purified Protein A is supplied as either 1.0 mg or 5.0 mg in lyophilized form. No BSA, preservatives or amine-containing buffer salts have been added. Store up to one year at -20 degree C; or after reconstitution in phosphate buffered saline to highest concentration possible, store up to one month at 2-8 degree C.
Protein A-HRP conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No other preservatives have been added. Store at 2-8 degree C or long-term at -20 degree C.
Reagents are stable for the period shown on the label if stored as directed.
Protein A-HRP conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No other preservatives have been added. Store at 2-8 degree C or long-term at -20 degree C.
Reagents are stable for the period shown on the label if stored as directed.
References
1. Kronvall G., U.S. Seal, J. Finstad, and R.C. Williams Jr. 1970. J. Immunol. 104:140.
2. Bjork I., B.A. Petersson, and J. Sjoquist. 1972. Eur. J. Biochem. 29:579.
3. Sjoquist J., B. Meloun, and H. Hjelm. 1972. Eur. J. Biochem. 29:572.
4. Ankerst J., P. Christensen, L. Kjellen, and G. Kronval. 1974. J. Infect. Dis. 130:268.
5. Kronvall G., U.S. Seal, S. Svensson, and R.C. Williams Jr. 1974. Acta. Pathol. Microbiol. Scand. [B] Microbiol. Immunol. 82:12.
6. Jonsson S., and G. Kronvall. 1974. Eur. J. Immunol. 4:29.
7. Hjelm H., J. Sjodahl, and J. Sjoquist. 1975. Eur. J. Biochem. 57:395.
8. Hjelm H. 1975. Scand. J. Immunol. 4:633.
9. Dubois-Dalcq M., H. McFarland, and D. McFarlin. 1977. J. Histochem. Cytochem. 25:1201.
10. Ey P.L., S.J. Prowse, C.R. Jenkin. 1978. Immunochemistry 15:429.
11. Langone J.J. 1978. J. Immunol. Methods 24:269.
12. Richman D.D., P.H. Cleveland, M.N. Oxman, and K.M. Johnson. 1982. J. Immunol. 128:2300.
13. Lindmark R., K. Thoren-Tolling, and J. Sjoquist. 1983. J. Immunol. Methods 62:1.
14. Chang H.C., I. Takashima, J. Arikawa, and N. Hashimoto. 1984. J. Virol. Methods 9:143.
15. Bjercke R.J., G. Cook, N. Rychlik, H.B. Gjika, H. Van Vunakis, and J.J. Langone. 1986. J. Immunol. Methods 90:203.
16. Compton B.J., M.A. Lewis, F. Whigham, J.S. Gerald, and G.E. Countryman. 1989. Anal. Chem. 61:1314.
17. Crichton D.N., and B.B. Cohen. 1989. Immunol. Today 10:325.
18. Watson P.R., T.H. Guthrie Jr., and R.J. Caruana. 1989. Cancer 64:1400.
19. Dean E.A., S.C. Whipp, and H.W. Moon. 1989. Infect. Immun. 57:82.
2. Bjork I., B.A. Petersson, and J. Sjoquist. 1972. Eur. J. Biochem. 29:579.
3. Sjoquist J., B. Meloun, and H. Hjelm. 1972. Eur. J. Biochem. 29:572.
4. Ankerst J., P. Christensen, L. Kjellen, and G. Kronval. 1974. J. Infect. Dis. 130:268.
5. Kronvall G., U.S. Seal, S. Svensson, and R.C. Williams Jr. 1974. Acta. Pathol. Microbiol. Scand. [B] Microbiol. Immunol. 82:12.
6. Jonsson S., and G. Kronvall. 1974. Eur. J. Immunol. 4:29.
7. Hjelm H., J. Sjodahl, and J. Sjoquist. 1975. Eur. J. Biochem. 57:395.
8. Hjelm H. 1975. Scand. J. Immunol. 4:633.
9. Dubois-Dalcq M., H. McFarland, and D. McFarlin. 1977. J. Histochem. Cytochem. 25:1201.
10. Ey P.L., S.J. Prowse, C.R. Jenkin. 1978. Immunochemistry 15:429.
11. Langone J.J. 1978. J. Immunol. Methods 24:269.
12. Richman D.D., P.H. Cleveland, M.N. Oxman, and K.M. Johnson. 1982. J. Immunol. 128:2300.
13. Lindmark R., K. Thoren-Tolling, and J. Sjoquist. 1983. J. Immunol. Methods 62:1.
14. Chang H.C., I. Takashima, J. Arikawa, and N. Hashimoto. 1984. J. Virol. Methods 9:143.
15. Bjercke R.J., G. Cook, N. Rychlik, H.B. Gjika, H. Van Vunakis, and J.J. Langone. 1986. J. Immunol. Methods 90:203.
16. Compton B.J., M.A. Lewis, F. Whigham, J.S. Gerald, and G.E. Countryman. 1989. Anal. Chem. 61:1314.
17. Crichton D.N., and B.B. Cohen. 1989. Immunol. Today 10:325.
18. Watson P.R., T.H. Guthrie Jr., and R.J. Caruana. 1989. Cancer 64:1400.
19. Dean E.A., S.C. Whipp, and H.W. Moon. 1989. Infect. Immun. 57:82.
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Product Notes
The Protein A faec (Catalog #AAA675011) is a Secondary Antibody produced from Staphylococcus aureus and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Protein A can be used in a range of immunoassay formats including, but not limited to, ELISA; Immunoblot; Immunohistochemistry; Immunoprecipitation. Researchers should empirically determine the suitability of the Protein A faec for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein A, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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