Rabbit anti-Human Proprotein Convertase Subtilisin Kexin 9 Polyclonal Antibody | anti-PCSK9 antibody

Proprotein Convertase Subtilisin Kexin 9, NT (Proprotein Convertase Subtilisin/kexin Type 9, PCSK9, FH3, Hypercholesterolemia Autosomal Dominant 3, HCHOLA3, LDLCQ1, Neural Apoptosis Regulated Convertase 1, NARC1, NARC-1, PC9, Proprotein Convertase PC9, PS

Cat. Num. AAA6494728

• Gene Names: PCSK9; FH3; PC9; FHCL3; NARC1; LDLCQ1; NARC-1; HCHOLA3
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Proprotein Convertase Subtilisin Kexin 9; Polyclonal Antibody; NT (Proprotein Convertase Subtilisin/kexin Type 9; PCSK9; FH3; Hypercholesterolemia Autosomal Dominant 3; HCHOLA3; LDLCQ1; Neural Apoptosis Regulated Convertase 1; NARC1; NARC-1; PC9; Proprotein Convertase PC9; PS; EC=3.4.21.-; anti-PCSK9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Proprotein Convertase Subtilisin Kexin 9.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
• Sequence Length: 692

• Applicable Applications for anti-PCSK9 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 153-183 in the N-terminal region of human Proprotein Convertase Subtilisin Kexin 9.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6006719. PCSK9 (arrow) was detected usingMBS6006719.)

Western Blot (WB)

(Western Blot analysis of lysates from HepG2, Hela, SW480 cell line (from left to right, 20ug/lane), using MBS6006719 (1:1000/lane). An IgG (HRP) goat anti-rabbit at 1:10,000 was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6006719 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Jurkat cells using MBS6006719 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PCSK9 antibody

Antibodies; Enzymes

References

1. Abifadel, M., Rabes, J.P. Hum. Mutat. 30(7), E682-E691 (2009). 2. McNutt, M.C., Kwon, H.J. J. Biol. Chem. 284(16), 10561-10570 (2009). 3. Shioji, K., Mannami, T. J. Hum. Genet. 49(2), 109-114 (2004). 4. Abifadel, M., Varret, M. Nat. Genet. 34(2), 154-156 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 31317307
• NCBI GeneID: 255738
• NCBI Accession #: NP_777596
• NCBI GenBank Nucleotide #: NP_777596.2
• UniProt Accession #: Q8NBP7
• Molecular Weight: 74.372
• NCBI Official Full Name: proprotein convertase subtilisin/kexin type 9 preproprotein
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 9
• NCBI Official Symbol: PCSK9
• NCBI Official Synonym Symbols: FH3; PC9; FHCL3; NARC1; LDLCQ1; NARC-1; HCHOLA3
• NCBI Protein Information: proprotein convertase subtilisin/kexin type 9
• UniProt Protein Name: Proprotein convertase subtilisin/kexin type 9
• Protein Family: Proprotein convertase subtilisin/kexin
• UniProt Gene Name: PCSK9
• UniProt Synonym Gene Names: NARC1; NARC-1; PC9
• UniProt Entry Name: PCSK9_HUMAN

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

PCSK9: Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. Defects in PCSK9 are the cause of hypercholesterolemia autosomal dominant type 3 (HCHOLA3). A familial condition characterized by elevated circulating cholesterol contained in either low-density lipoproteins alone or also in very-low-density lipoproteins. Belongs to the peptidase S8 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Secreted; Protease; Secreted, signal peptide; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: Golgi apparatus; extracellular space; cell surface; extrinsic to external side of plasma membrane; rough endoplasmic reticulum; endoplasmic reticulum; lysosome; perinuclear region of cytoplasm; early endosome; cytoplasm; late endosome; plasma membrane; ER to Golgi transport vesicle

Molecular Function: sodium channel inhibitor activity; very-low-density lipoprotein binding; protein binding; protein self-association; low-density lipoprotein receptor binding; serine-type endopeptidase activity; low-density lipoprotein binding; apolipoprotein binding; apolipoprotein receptor binding

Biological Process: cholesterol metabolic process; lysosomal transport; apoptosis; positive regulation of receptor internalization; lipoprotein metabolic process; regulation of low-density lipoprotein receptor catabolic process; proteolysis; liver development; cellular response to starvation; neuron differentiation; protein autoprocessing; cholesterol homeostasis; triacylglycerol metabolic process; cellular response to insulin stimulus; neurogenesis; phospholipid metabolic process; positive regulation of neuron apoptosis; regulation of neuron apoptosis; negative regulation of receptor recycling; low-density lipoprotein receptor catabolic process; regulation of receptor activity; kidney development

Disease: Hypercholesterolemia, Autosomal Dominant, 3