Western Blot (WB)
(Anti-Proprotein convertase PC4 antibody, MBS176322, Western blottingAll lanes: Anti Proprotein convertase PC4 (MBS176322) at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 83KDObserved bind size: 83KD)
Rabbit anti-Mouse, Rat Proprotein convertase PC4 Polyclonal Antibody | anti-PCSK4 antibody
Anti-Proprotein convertase PC4 antibody
Gene Names
Pcsk4; PC4; NEC3; SPC5; NEC 3; L21221; AI647044
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Proprotein convertase PC4; Polyclonal Antibody; Anti-Proprotein convertase PC4 antibody; Proprotein convertase subtilisin/kexin type 4; proprotein convertase subtilisin/kexin type 4; PC4 antibody; PCSK4 antibody; PCSK4_HUMAN antibody; Proprotein convertase 4 antibody; Proprotein convertase PC4 antibody; Proprotein convertase subtilisin/kexin type 4 antibody; SPC5 antibody; anti-PCSK4 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
655
Applicable Applications for anti-PCSK4 antibody
Western Blot (WB)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Proprotein convertase PC4 (117-133aa DPWFSKQWYMNKEIQQD), different from the related rat sequence by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-Proprotein convertase PC4 antibody, MBS176322, Western blottingAll lanes: Anti Proprotein convertase PC4 (MBS176322) at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 83KDObserved bind size: 83KD)
Western Blot (WB)
(Anti-Proprotein convertase PC4 antibody, MBS176322, Western blottingAll lanes: Anti Proprotein convertase PC4 (MBS176322) at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 83KDObserved bind size: 83KD)
Related Product Information for anti-PCSK4 antibody
Description: Rabbit IgG polyclonal antibody for Proprotein convertase subtilisin/kexin type 4(PCSK4) detection. Tested with WB in Mouse, Rat.
Background: PCSK4(Proprotein Convertase, Subtilisin/Kexin-Type, 4), is an enzyme that in humans is encoded by the PCSK4 gene. The gene for PC4(Pcsk4 locus) was mapped to mouse chromosome 10, close to the adipsin locus and near the gene encoding anti-mullerian hormone. Qiu et al.(2005) found that aberrant processing of pro-IGF2 by PC4 may be a cause of IUGR. PC4 cleaved pro-IGF2 to generate the intermediate processed form, IGF2(1-102). Mature IGF2(1-67), likely resulting from further processing by carboxypeptidases, is capable of activating invasive trophoblast cells through AKT phosphorylation. Inhibition of PC4 by a PC4-specific inhibitor blocked pro-IGF2 processing and reduced trophoblast cell migration.
Background: PCSK4(Proprotein Convertase, Subtilisin/Kexin-Type, 4), is an enzyme that in humans is encoded by the PCSK4 gene. The gene for PC4(Pcsk4 locus) was mapped to mouse chromosome 10, close to the adipsin locus and near the gene encoding anti-mullerian hormone. Qiu et al.(2005) found that aberrant processing of pro-IGF2 by PC4 may be a cause of IUGR. PC4 cleaved pro-IGF2 to generate the intermediate processed form, IGF2(1-102). Mature IGF2(1-67), likely resulting from further processing by carboxypeptidases, is capable of activating invasive trophoblast cells through AKT phosphorylation. Inhibition of PC4 by a PC4-specific inhibitor blocked pro-IGF2 processing and reduced trophoblast cell migration.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
70,203 Da
NCBI Official Full Name
Proprotein convertase subtilisin/kexin type 4
NCBI Official Synonym Full Names
proprotein convertase subtilisin/kexin type 4
NCBI Official Symbol
Pcsk4
NCBI Official Synonym Symbols
PC4; NEC3; SPC5; NEC 3; L21221; AI647044
NCBI Protein Information
proprotein convertase subtilisin/kexin type 4; prohormone convertase 3; KEX2-like endoprotease 3; neuroendocrine convertase 3
UniProt Protein Name
Proprotein convertase subtilisin/kexin type 4
Protein Family
UniProt Gene Name
Pcsk4
UniProt Synonym Gene Names
Nec-3; Nec3; NEC 3
UniProt Entry Name
PCSK4_MOUSE
Uniprot Description
PCSK4: Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Plays a role in transcriptional coactivation. May be involved in stabilizing the multiprotein transcription complex. Belongs to the peptidase S8 family. Furin subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.21.-; Membrane protein, integral; Protease; Cell development/differentiation
Cellular Component: cell; acrosomal membrane
Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: fertilization; binding of sperm to zona pellucida; acrosome reaction; proteolysis; sperm capacitation
Research Articles on PCSK4
Similar Products
Product Notes
The PCSK4 pcsk4 (Catalog #AAA176322) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Proprotein convertase PC4 antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Proprotein convertase PC4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the PCSK4 pcsk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Proprotein convertase PC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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