Rat Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) ELISA Kit | PCSK1 elisa kit

Rat Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) ELISA Kit

Cat. Num. AAA2021585

• Gene Names: PCSK1; PC1; PC3; NEC1; SPC3; BMIQ12
• Reactivity: Rat
• Synonyms: Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1); Rat Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) ELISA Kit; PC1; PC3; SPC3; NEC1; Prohormone Convertase 3; Prohormone Convertase 1; Neuroendocrine Convertase 1; Proprotein Convertase 1; PCSK1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1).; No significant cross-reactivity or interference between Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) and analogues was observed.
• Sequence Length: 706

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.44ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCSK1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PCSK1 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 223462732
• NCBI GeneID: 5122
• UniProt Accession #: P29120; P78478; Q92532; B7Z8T7; E9PHA1
• Molecular Weight: 79,010 Da
• NCBI Official Full Name: Proprotein convertase subtilisin/kexin type 1
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 1
• NCBI Official Symbol: PCSK1
• NCBI Official Synonym Symbols: PC1; PC3; NEC1; SPC3; BMIQ12
• NCBI Protein Information: neuroendocrine convertase 1
• UniProt Protein Name: Neuroendocrine convertase 1
• Protein Family: Neuroendocrine convertase
• UniProt Gene Name: PCSK1
• UniProt Synonym Gene Names: NEC1; NEC 1; PC1
• UniProt Entry Name: NEC1_HUMAN

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. The protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Mutations in this gene have been associated with susceptibility to obesity and proprotein convertase 1/3 deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene [provided by RefSeq, Jan 2014]

Uniprot Description

PCSK1: Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Belongs to the peptidase S8 family. Furin subfamily.

Protein type: EC 3.4.21.93; Protease

Chromosomal Location of Human Ortholog: 5q15-q21

Cellular Component: Golgi apparatus; extracellular space; transport vesicle

Molecular Function: serine-type endopeptidase activity

Biological Process: cellular protein metabolic process; cell-cell signaling; metabolic process; peptide hormone processing; proteolysis; regulation of insulin secretion; peptide biosynthetic process

Disease: Proprotein Convertase 1/3 Deficiency; Body Mass Index Quantitative Trait Locus 12