Rabbit anti-Human, Mouse Proline Dehydrogenase Polyclonal Antibody | anti-PRODH antibody

Proline Dehydrogenase, ID (PRODH, HSPOX2, P53-induced Gene 6 Protein, PIG6, PRODH1, PRODH2, Proline Dehydrogenase (Oxidase) 1, Proline Dehydrogenase (Proline Oxidase), Proline Oxidase Mitochondrial Precursor, SCZD4, Tumor Protein p53 Inducible Protein 6,

Cat. Num. AAA6494667

• Gene Names: PRODH; POX; PIG6; HSPOX2; PRODH1; PRODH2; TP53I6
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Proline Dehydrogenase; Polyclonal Antibody; ID (PRODH; HSPOX2; P53-induced Gene 6 Protein; PIG6; PRODH1; PRODH2; Proline Dehydrogenase (Oxidase) 1; Proline Dehydrogenase (Proline Oxidase); Proline Oxidase Mitochondrial Precursor; SCZD4; Tumor Protein p53 Inducible Protein 6; ; anti-PRODH antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRODH. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PRODH antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human PRODH (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of mouse Prodh Antibody (Center) in mouse liver, kidney tissue lysates (35ug/lane). PRODH (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with mouse Prodh Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of ATDC5 cells using mouse Prodh Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PRODH antibody

Antibodies; Enzymes; Oxidase

References

1. Polyak, K., et.al., Nature 389(6648), 300-305 (1997). 2. Gogos, J.A., et.al., Nat. Genet. 21(4), 434-439 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 183227691
• NCBI GeneID: 5625
• NCBI Accession #: NP_057419.3
• NCBI GenBank Nucleotide #: NM_016335.3
• UniProt Accession #: O43272; O14680; Q0P507; Q147W8; Q504W1; Q59FI8; Q6NV86; Q9UF13; A6NF53
• Molecular Weight: 59,231 Da
• NCBI Official Full Name: proline dehydrogenase, mitochondrial
• NCBI Official Synonym Full Names: proline dehydrogenase (oxidase) 1
• NCBI Official Symbol: PRODH
• NCBI Official Synonym Symbols: POX; PIG6; HSPOX2; PRODH1; PRODH2; TP53I6
• NCBI Protein Information: proline dehydrogenase 1, mitochondrial; proline oxidase 2; p53-induced gene 6 protein; proline oxidase, mitochondrial; tumor protein p53 inducible protein 6
• UniProt Protein Name: Proline dehydrogenase 1, mitochondrial
• Protein Family: Proline dehydrogenase
• UniProt Gene Name: PRODH
• UniProt Synonym Gene Names: PIG6; POX2
• UniProt Entry Name: PROD_HUMAN

NCBI Description

This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

PRODH: Converts proline to delta-1-pyrroline-5-carboxylate. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1). HP-1 is a disorder characterized by elevated serum proline levels. May be involved in the psychiatric and behavioral phenotypes associated with the 22q11 velocardiofacial and DiGeorge syndrome. Defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. Belongs to the proline oxidase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; Oxidoreductase; Mitochondrial; EC 1.5.5.2

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: mitochondrial matrix; mitochondrial inner membrane

Molecular Function: proline dehydrogenase activity

Biological Process: proline catabolic process; 4-hydroxyproline catabolic process; induction of apoptosis by oxidative stress; proline catabolic process to glutamate; proline metabolic process

Disease: Hyperprolinemia, Type I; Schizophrenia 4