Rabbit anti-Human Prolactin Receptor, Intermediate isoform Polyclonal Antibody | anti-PRLR antibody

Prolactin Receptor, Intermediate isoform (PRLR, PRL-R, PRL Receptor)

Cat. Num. AAA611410

• Gene Names: PRLR; hPRLrI
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Prolactin Receptor; Intermediate isoform; Polyclonal Antibody; Intermediate isoform (PRLR; PRL-R; PRL Receptor); Anti -Prolactin Receptor; anti-PRLR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the unique sequence of the human prolactin receptor (PRLr) intermediate isoform. Reactivity is confirmed with T47D breast carcinoma cell line.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-PRLR antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1-3ug/ml; Immunopreciptation: 10ug/IP reaction
• Immunogen: Synthetic peptide derived from the C-terminal sequence of human prolactin receptor intermediate isoform.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-PRLR antibody

Prolactin (PRL) is a neuroendocrine hormone that is closely related to growth hormones. The endocrine effects of PRL on human breast tissues include the regulation of growth and differentiation of ductal epithelium, proliferation and differentiation of lobular units, and initiation and maintenance of lactation.. Both the PRLr and growth hormone receptors are single-chain transmembrane proteins composed of extracellular, transmembrane, and intracellular domains. Prolactin modulates immune system function by stimulating the proliferation and survival of cells through its receptor, present on T and B lymphocytes and macrophages. The function of PRL is mediated by a variety of signaling cascades as well as by the wide variety of PRLr isoforms observed in nature. A long form and several other isoforms are expressed in human tissues. The nucleotide sequence of the novel intermediate isoform of PRLr is identical to the long isoform, except for a 573-base pair deletion occurring at a consensus splice site, resulting in a frameshift and truncated intracytoplasmic domain. The long isoform of PRLr is N-glycosylated. As the extracellular domain of the intermediate isoform is identical to that of the long isoform, glycosylation patterns are assumed to be similar. Using immunohistochemical analysis, PRLr expression has been observed in 95% of both normal and malignant breast epithelium; no correlation between PRL expression and ER-PR status has been established.

Product Categories/Family for anti-PRLR antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 861449
• NCBI GeneID: 5618
• UniProt Accession #: Q16264; P16471; Q8TD76; Q8WWV7
• Molecular Weight: 5,273 Da
• NCBI Official Full Name: prolactin receptor intermediate isoform
• NCBI Official Synonym Full Names: prolactin receptor
• NCBI Official Symbol: PRLR
• NCBI Official Synonym Symbols: hPRLrI
• NCBI Protein Information: prolactin receptor; PRL-R; hPRL receptor; OTTHUMP00000115998; OTTHUMP00000221070; OTTHUMP00000221071; OTTHUMP00000221073; secreted prolactin binding protein; delta 4-SF1b truncated prolactin receptor; delta 4-delta 7/11 truncated prolactin receptor
• UniProt Protein Name: Prolactin receptor intermediate isoform
• Protein Family: Prolactin receptor
• UniProt Entry Name: Q16264_HUMAN

NCBI Description

This gene encodes a receptor for the anterior pituitary hormone, prolactin, and belongs to the type I cytokine receptor family. Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of the prolactin receptor. Several alternatively spliced transcript variants encoding different membrane-bound and soluble isoforms have been described for this gene, which may function to modulate the endocrine and autocrine effects of prolactin in normal tissue and cancer. [provided by RefSeq]

Uniprot Description

PRLR: receptor for the anterior pituitary hormone prolactin and placental lactogen I and II. Interacts with Cyclophilin A, differentially regulating various signaling pathways from the PrlR. Prolactin signaling is attenuated by threonine and tyrosine phosphorylation. Two alternative splice isoforms have been identified.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p13.2

Cellular Component: cell surface; plasma membrane; extracellular region; integral to membrane

Molecular Function: protein binding; protein homodimerization activity; metal ion binding; ornithine decarboxylase activator activity; peptide hormone binding; prolactin receptor activity

Biological Process: lactation; regulation of cell adhesion; transmembrane receptor protein tyrosine kinase activation (dimerization); cell surface receptor linked signal transduction; T cell activation; regulation of epithelial cell differentiation; tyrosine phosphorylation of JAK2 protein; steroid biosynthetic process; embryo implantation; negative regulation of apoptosis

Disease: Multiple Fibroadenomas Of The Breast; Hyperprolactinemia