Rabbit anti-Rat Prolactin Receptor Polyclonal Antibody | anti-PRLR antibody

Anti-Rat Prolactin Receptor, Purified, (polyclonal), (Rabbit IgG)

Cat. Num. AAA524150

• Gene Names: PRLR; hPRLrI
• Reactivity: Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: Prolactin Receptor; Polyclonal Antibody; Anti-Rat Prolactin Receptor; Purified; (polyclonal); (Rabbit IgG); Purified Rabbit anti-Mouse/Rat Prolactin Receptor Polyclonal Antibody; anti-PRLR antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Prolactin Receptor (This antibody is specific for the rat and mouse prolactin receptor)
• Form/Format: Purified
• Sequence Length: 622

• Applicable Applications for anti-PRLR antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry Paraffin (IHC)
• Application Notes: Suggested minimum working dilution of 1:500 in Western Blot; 1:1000 in ELISA and 1:1000 in IHC-P. This antibody has not been tested in other applications.
• Immunogen: 16 amino acid synthetic peptide in the cytoplasmic domain of rat isoform 3 and mouse CRA-b isoform.
• Presentation: 100 ug purified IgG buffered in 100 ul PBS and 0.02% NaN3 (1 mg/ml). (Purified from serum via Affinity Chromatography). For maximum recovery of contents, spin down tube before use.
• Preparation and Storage: Store at + 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Related Product Information for anti-PRLR antibody

Prolactin (PRL) is a pituitary hormone involved in a variety of essential functions including reproduction, ion transport, growth, development and immunoregulation. It is also classified as a cytokine and influences different cell types through a signal transduction pathway upon ligand binding and dimerization of the Prolactin Receptor. The transmembrane PRL receptor, a member of the cytokine receptor superfamily, can be found in long and short forms depending on tissue source and species (ranging from ~40 kDa to 100 kDa). Signal transduction is mediated with the aid of members of the JAK/STAT signaling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 4506107
• NCBI GeneID: 5618
• NCBI Accession #: NP_000940.1
• NCBI GenBank Nucleotide #: NM_000949.5
• UniProt Accession #: P16471; Q16354; Q8TD75; Q8TD78; Q96P35; Q96P36; Q9BX87; Q9UHJ5; B2R882; D1MDP1
• Molecular Weight: 69,506 Da
• NCBI Official Full Name: prolactin receptor isoform 1
• NCBI Official Synonym Full Names: prolactin receptor
• NCBI Official Symbol: PRLR
• NCBI Official Synonym Symbols: hPRLrI
• NCBI Protein Information: prolactin receptor; PRL-R; hPRL receptor; secreted prolactin binding protein
• UniProt Protein Name: Prolactin receptor
• Protein Family: Prolactin receptor
• UniProt Gene Name: PRLR
• UniProt Synonym Gene Names: PRL-R
• UniProt Entry Name: PRLR_HUMAN

NCBI Description

This gene encodes a receptor for the anterior pituitary hormone, prolactin, and belongs to the type I cytokine receptor family. Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of the prolactin receptor. Several alternatively spliced transcript variants encoding different membrane-bound and soluble isoforms have been described for this gene, which may function to modulate the endocrine and autocrine effects of prolactin in normal tissue and cancer. [provided by RefSeq, Feb 2011]

Uniprot Description

Function: This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling. Ref.6 Ref.7

Subunit structure: Homodimer upon hormone binding. Interacts with SMARCA1. Interacts with GH1. Interacts with CSH. Interacts with NEK3 and VAV2 and this interaction is prolactin-dependent. Ref.4 Ref.13 Ref.14 Ref.15 Ref.17

Subcellular location: Membrane; Single-pass type I membrane protein Ref.4 Ref.6. Isoform 7: Secreted Ref.4 Ref.6.

Tissue specificity: Expressed in breast, placenta, kidney, liver and pancreas. Ref.4 Ref.6

Domain: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The box 1 motif is required for JAK interaction and/or activation.

Sequence similarities: Belongs to the type I cytokine receptor family. Type 1 subfamily.Contains 2 fibronectin type-III domains.