Rabbit HIP1 Polyclonal Antibody | anti-HIP1 antibody

HIP1 Antibody

Cat. Num. AAA9435385

• Gene Names: HIP1; HIP-I; ILWEQ
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide affinity purified.
• Synonyms: HIP1; Polyclonal Antibody; HIP1 Antibody; 2610109B09Rik antibody; A930014B11Rik antibody; E130315I21Rik antibody; HIP 1 antibody; HIP I antibody; HIP-1 antibody; HIP-I antibody; hip1 antibody; HIP1/PDGFRB fusion gene antibody; HIP1/PDGFRB fusion gene; included antibody; HIP1_HUMAN antibody; HIPI antibody; Huntingtin interacting protein 1 antibody; Huntingtin-interacting protein 1 antibody; Huntingtin-interacting protein I antibody; ILWEQ antibody; KIAA4113 antibody; MGC126506 antibody; MGC27616 antibody; mKIAA4113 antibody; anti-HIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Peptide affinity purified.
• Form/Format: 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-HIP1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; ICC: 1:100-1:500; FC/FACS: 1:50-1:100
• Immunogen: Synthetic peptide within C terminal human Hip1.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Hip1 on different tissue lysates using anti-Hip1 antibody at 1/500 dilution. Positive control�� Lane1: Mouse testis Lane2: Mouse spinal cord Lane3: SH-SY5Y)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hip1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hip1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-Hip1 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Hip1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Hip1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Hip1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of SH-SY5Y cells with Hip1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Related Product Information for anti-HIP1 antibody

Huntington disease is associated with the expansion of a polyglutamine tract, greater than 35 repeats, in the HD gene product huntingtin. HIP1 (huntingtin-interacting protein 1), a membrane-associated protein, binds specifically to the N-terminus of human huntingtin. HIP1 is ubiquitously expressed in different brain regions at low levels, and exhibits nearly identical subcellular fractionation as huntingtin. The huntingtin-HIP1 interaction is restricted to the brain and is inversely correlated to the polyglutamine length in the huntingtin, suggesting that loss of normal huntingtin-HIP1 interaction may compromise the membrane-cytoskeletal integrity in the brain. HIP1 contains an endocytic multidomain protein with a C-terminal Actin-binding domain, a central coiled-coil forming region and an N-terminal ENTH domain. HIP1 may be involved in vesicle trafficking; the structural integrity of HIP1 is crucial for maintenance of normal vesicle size in vivo. HIP12 is a non-proapoptotic member of the HIP gene family that is expressed in the brain and shares a similar subcellular distribution pattern with HIP1. However, HIP12 differs from HIP1 in its pattern of expression at both the mRNA and protein level. HIP12 does not directly interact with huntingtin but can interact with HIP1.

Product Categories/Family for anti-HIP1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 206729937
• NCBI GeneID: 3092
• UniProt Accession #: O00291; O00328; Q2TB58; Q8TDL4; B4E3I7; E7ES17
• Molecular Weight: 1037
• NCBI Official Full Name: Huntingtin-interacting protein 1
• NCBI Official Synonym Full Names: huntingtin interacting protein 1
• NCBI Official Symbol: HIP1
• NCBI Official Synonym Symbols: HIP-I; ILWEQ
• NCBI Protein Information: huntingtin-interacting protein 1; huntingtin-interacting protein I
• UniProt Protein Name: Huntingtin-interacting protein 1
• Protein Family: Histidine permease
• UniProt Gene Name: HIP1
• UniProt Synonym Gene Names: HIP-1; HIP-I
• UniProt Entry Name: HIP1_HUMAN

NCBI Description

The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

HIP1: Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR)-mediated transcription. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. May play a functional role in the cell filament networks. May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors. A chromosomal aberration involving HIP1 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;7)(q33;q11.2) with PDGFRB. The chimeric HIP1-PDGFRB transcript results from an in-frame fusion of the two genes. The reciprocal PDGFRB-HIP1 transcript is not expressed. Belongs to the SLA2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Apoptosis

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: Golgi apparatus; cytoskeleton; intracellular membrane-bound organelle; clathrin-coated vesicle; membrane; cytoplasm; clathrin coated vesicle membrane; nucleus

Molecular Function: protein binding, bridging; clathrin binding; protein binding; structural constituent of cytoskeleton; actin binding; phosphoinositide binding

Biological Process: regulation of apoptosis; caspase activation; clathrin cage assembly; transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; endocytosis; cell differentiation; actin cytoskeleton organization and biogenesis; positive regulation of receptor-mediated endocytosis

Disease: Prostate Cancer