Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Procollagen C Proteinase Enhancer 1 (PCPE1) Polyclonal Antibody | anti-PCPE1 antibody
Polyclonal Antibody to Procollagen C Proteinase Enhancer 1 (PCPE1)
Gene Names
Pcolce; P14; Astt2; Astt-2; PCPE-1
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Procollagen C Proteinase Enhancer 1 (PCPE1); Polyclonal Antibody; Polyclonal Antibody to Procollagen C Proteinase Enhancer 1 (PCPE1); anti-PCPE1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
FSupplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol.
Concentration
0.31mg/ml (varies by lot)
Applicable Applications for anti-PCPE1 antibody
WB; IHC; ICC; IP.
Application Notes
Western blotting: 0.5-2 ug/mL;1:150-600
Immunohistochemistry: 5-20 ug/mL;1:15-60
Immunocytochemistry: 5-20 ug/mL;1:15-60
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20 ug/mL;1:15-60
Immunocytochemistry: 5-20 ug/mL;1:15-60
Optimal working dilutions must be determined by end user.
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant PCPE1 (Cys36~Ala468 (Accession # Q61398)) expressed in E.coli
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2053836
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Heart tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Heart tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
procollagen C-endopeptidase enhancer 1
NCBI Official Synonym Full Names
procollagen C-endopeptidase enhancer protein
NCBI Official Symbol
Pcolce
NCBI Official Synonym Symbols
P14; Astt2; Astt-2; PCPE-1
NCBI Protein Information
procollagen C-endopeptidase enhancer 1
UniProt Protein Name
Procollagen C-endopeptidase enhancer 1
UniProt Gene Name
Pcolce
UniProt Synonym Gene Names
Pcpe1; PCPE-1; Procollagen C-proteinase enhancer 1
Uniprot Description
PCOLCE: Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.
Protein type: Extracellular matrix; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5 G2|5 76.59 cM
Cellular Component: extracellular exosome; extracellular matrix; extracellular region; extracellular space
Molecular Function: collagen binding; heparin binding; protease activator activity
Biological Process: proteolysis
Research Articles on PCPE1
Similar Products
Product Notes
The PCPE1 pcolce (Catalog #AAA2004251) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Procollagen C Proteinase Enhancer 1 (PCPE1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Procollagen C Proteinase Enhancer 1 (PCPE1) can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2 ug/mL;1:150-600 Immunohistochemistry: 5-20 ug/mL;1:15-60 Immunocytochemistry: 5-20 ug/mL;1:15-60 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PCPE1 pcolce for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Procollagen C Proteinase Enhancer 1 (PCPE1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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