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Western Blot (WB) (Western Blot: Sample: Mouse Pancreas Tissue.)

Rabbit anti-Human Procalcitonin (PCT) Polyclonal Antibody | anti-PCT antibody

Polyclonal Antibody to Procalcitonin (PCT)

Gene Names
CALCA; CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Procalcitonin (PCT); Polyclonal Antibody; Polyclonal Antibody to Procalcitonin (PCT); Pro-Calcitonin; anti-PCT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PCT. It has been selected for its ability to recognize PCT in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid
Concentration
0.45mg/ml (varies by lot)
Sequence Length
141
Applicable Applications for anti-PCT antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.5-5ug/mL
Immunocytochemistry in formalin fixed cells: 5-20ug/mL
Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL
Immunohistochemistry in paraffin section: 5-20ug/mL
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Cross Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Mouse Pancreas Tissue.)

Western Blot (WB) (Western Blot: Sample: Mouse Pancreas Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant PCT, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant PCT, Human.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
796
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,898 Da
NCBI Official Full Name
calcitonin isoform CT preproprotein
NCBI Official Synonym Full Names
calcitonin related polypeptide alpha
NCBI Official Symbol
CALCA
NCBI Official Synonym Symbols
CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
NCBI Protein Information
calcitonin; calcitonin gene-related peptide 1
UniProt Protein Name
Calcitonin
UniProt Gene Name
CALCA
UniProt Synonym Gene Names
CALC1; CCP

NCBI Description

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]

Uniprot Description

CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: cell soma; cytoplasm; extracellular region; extracellular space; nucleus; terminal bouton

Molecular Function: calcitonin receptor binding; hormone activity; identical protein binding; protein binding

Biological Process: activation of protein kinase activity; adenylate cyclase activation; adenylate cyclase-activating G-protein coupled receptor signaling pathway; aging; cellular protein metabolic process; detection of temperature stimulus involved in sensory perception of pain; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); embryo implantation; feeding behavior; inflammatory response; monocyte chemotaxis; negative regulation of bone resorption; negative regulation of neurological process; negative regulation of ossification; negative regulation of smooth muscle contraction; negative regulation of transcription, DNA-dependent; neuropeptide signaling pathway; positive regulation of adenylate cyclase activity; positive regulation of ossification; regulation of heart rate; response to heat; response to pain; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure

Research Articles on PCT

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Product Notes

The PCT calca (Catalog #AAA2090471) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Procalcitonin (PCT) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Procalcitonin (PCT) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-5ug/mL Immunocytochemistry in formalin fixed cells: 5-20ug/mL Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL Immunohistochemistry in paraffin section: 5-20ug/mL. Researchers should empirically determine the suitability of the PCT calca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Procalcitonin (PCT), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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