Rabbit BAD Polyclonal Antibody | anti-BAD antibody

BAD (Ab-136) Antibody

Cat. Num. AAA9404157

• Gene Names: Bad; Bbc2; AI325008
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: BAD; Polyclonal Antibody; BAD (Ab-136) Antibody; Bbc2; anti-BAD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total BAD protein.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 204

• Applicable Applications for anti-BAD antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around aa.134~138 (S-R-S-A-P) derived from Mouse BAD.
• Target Name: BAD
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from HT29 and HepG2 cells using BAD(MBS9404157) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(MBS9404157) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Related Product Information for anti-BAD antibody

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Product Categories/Family for anti-BAD antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 6671610
• NCBI GeneID: 12015
• NCBI Accession #: NP_031548.1
• NCBI GenBank Nucleotide #: NM_007522.3
• UniProt Accession #: Q61337
• Molecular Weight: 23kd
• NCBI Official Full Name: bcl2-associated agonist of cell death isoform 1
• NCBI Official Synonym Full Names: BCL2-associated agonist of cell death
• NCBI Official Symbol: Bad
• NCBI Official Synonym Symbols: Bbc2; AI325008
• NCBI Protein Information: bcl2-associated agonist of cell death
• UniProt Protein Name: Bcl2-associated agonist of cell death
• Protein Family: Bcl2-associated agonist of cell death
• UniProt Gene Name: Bad
• UniProt Synonym Gene Names: Bbc6; BAD
• UniProt Entry Name: BAD_MOUSE

Uniprot Description

BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.

Protein type: Apoptosis

Cellular Component: mitochondrial outer membrane; mitochondrion; membrane; cytoplasm; intracellular; cytosol

Molecular Function: protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; protein phosphatase binding; lipid binding; protein kinase binding; protein phosphatase 2B binding

Biological Process: apoptosis; positive regulation of proteolysis; positive regulation of apoptosis; positive regulation of caspase activity; cellular process regulating host cell cycle in response to virus; regulation of caspase activity; glucose homeostasis; positive regulation of apoptosis by virus; regulation of apoptosis; pore complex biogenesis; positive regulation of glucokinase activity; caspase activation; release of cytochrome c from mitochondria; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; ATP metabolic process; glucose catabolic process; cell proliferation; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; positive regulation of T cell differentiation; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; positive regulation of epithelial cell proliferation