Mouse PRMT6 Monoclonal Antibody | anti-PRMT6 antibody

PRMT6 Mouse Monoclonal Antibody [Clone PCRP-PRMT6-2C9]

Cat. Num. AAA4382141

• Gene Names: PRMT6; HRMT1L6
• Reactivity: Human; Predicted to react with Rat.
• Applications: Flow Cytometry, Functional Assay
• Synonyms: PRMT6; Monoclonal Antibody; PRMT6 Mouse Monoclonal Antibody [Clone PCRP-PRMT6-2C9]; Chromobox protein homolog 7; Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6; Histone-arginine N-methyltransferase PRMT6; HMT1 hnRNP methyltransferase like 6. ; HRMT1L6; anti-PRMT6 antibody

• Host: Mouse
• Reactivity: Human; Predicted to react with Rat.
• Clonality: Monoclonal
• Isotype: Mouse/IgG2b
• Clone Number: PCRP-PRMT6-2C9
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-PRMT6 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: 1-2ug/million cells
• Immunogen: Recombinant full-length human PRMT6 protein
• Cellular Localization: Nucleus
• Positive Control: HeLa or HepG2 cells.
• Hu-chromosome Location: 1p13.3
• Unigene: 26006
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. PRMT6 Mouse Monoclonal Antibody (PCRP-PRMT6-2C9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )

Related Product Information for anti-PRMT6 antibody

A class of proteins termed type 1 protein arginine N-methyltransferase (PRMT) enzymes contribute to posttranslational modification of RNA-binding proteins, but differ in substrate specificities, oligomerization properties and subcellular localization. PRMTs contain an S-adenosylmethione motif which functions to add one or two methyl groups to guanidino nitrogens of arginine (R) side chains. PRMT6, also known as HRMT1L6, is a nuclear protein belonging to the PRMT family and is predominantly expressed in testis and kidney. It is known to methylate Histones H3, H4 and H2A. PRMT6 is the major dimethyltransferase for Histone H3 and specifically methylates Histone H3 at R2. Methylation at Histone H3 R2 acts to inhibit Histone H3 K4 trimethylation and ultimately leads to the transcriptional repression of genes that are activated by Histone H3 K4 trimethylation. In addition, PRMT6 methylates HIV TAT, possibly functioning as a form of cellular innate immunity to restrict levels of HIV replication.

NCBI and Uniprot Product Information

• NCBI GI #: 20137409
• NCBI GeneID: 55170
• UniProt Accession #: Q96LA8; Q5T5Y5; Q6DKI4; Q9NVR8; A3KME1; B4DID8
• Molecular Weight: 375
• NCBI Official Full Name: Protein arginine N-methyltransferase 6
• NCBI Official Synonym Full Names: protein arginine methyltransferase 6
• NCBI Official Symbol: PRMT6
• NCBI Official Synonym Symbols: HRMT1L6
• NCBI Protein Information: protein arginine N-methyltransferase 6; HMT1 hnRNP methyltransferase-like 6; histone-arginine N-methyltransferase PRMT6; heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6
• UniProt Protein Name: Protein arginine N-methyltransferase 6
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT6
• UniProt Synonym Gene Names: HRMT1L6
• UniProt Entry Name: ANM6_HUMAN

NCBI Description

The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq, Sep 2011]

Uniprot Description

PRMT6: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC). Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.125; Methyltransferase, protein arginine

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: histone methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; protein binding; histone binding

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; viral reproduction; transcription, DNA-dependent; base-excision repair; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of transcription, DNA-dependent