Rabbit anti-Human PRMT5 Polyclonal Antibody | anti-PRMT5 antibody

PRMT5, CT (Protein Methyltransferase JBP1, Protein Arginine N-methyltransferase 5, Histone-arginine N-methyltransferase PRMT5, Shk1 Kinase-binding Protein 1 Homolog, SKB1 Homolog, SKB1Hs, Jak-binding Protein 1, 72kD ICln-Binding Protein, HRMT1L5, IBP72, J

Cat. Num. AAA6494413

• Gene Names: PRMT5; HSL7; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PRMT5; Polyclonal Antibody; CT (Protein Methyltransferase JBP1; Protein Arginine N-methyltransferase 5; Histone-arginine N-methyltransferase PRMT5; Shk1 Kinase-binding Protein 1 Homolog; SKB1 Homolog; SKB1Hs; Jak-binding Protein 1; 72kD ICln-Binding Protein; HRMT1L5; IBP72; J; anti-PRMT5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRMT5.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 637

• Applicable Applications for anti-PRMT5 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PRMT5 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PRMT5 Pab is used in Western blot to detect PRMT5 in HL60 cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PRMT5 antibody

Antibodies; Enzymes; Methyltransferase

References

1. Pal, S., et al., Mol. Cell. Biol. 23(21):7475-7487 (2003). 2. Rho, J., et al., J. Biol. Chem. 276(14):11393-11401 (2001). 3. Pollack, B.P., et al., J. Biol. Chem. 274(44):31531-31542 (1999). 4. Gilbreth, M., et al., Proc. Natl. Acad. Sci. USA 95(25):14781-14786 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 6164704
• NCBI GeneID: 10419
• Molecular Weight: 72.683
• NCBI Official Full Name: protein methyltransferase
• NCBI Official Synonym Full Names: protein arginine methyltransferase 5
• NCBI Official Symbol: PRMT5
• NCBI Official Synonym Symbols: HSL7; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• NCBI Protein Information: protein arginine N-methyltransferase 5
• Protein Family: Protein arginine N-methyltransferase

NCBI Description

This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]