Human Cytoplasmic tyrosine-protein kinase BMX ELISA Kit | BMX elisa kit

Human Cytoplasmic tyrosine-protein kinase BMX ELISA Kit

Cat. Num. AAA763749

• Gene Names: BMX; ETK; PSCTK2; PSCTK3
• Reactivity: Human
• Synonyms: Cytoplasmic tyrosine-protein kinase BMX; Human Cytoplasmic tyrosine-protein kinase BMX ELISA Kit; BMX/ETK/NTK38/PSCTK3/BMX non-receptor tyrosine kinase/Bone marrow tyrosine kinase gene in chromosome X protein/Epithelial and endothelial tyrosine kinase/Etk/Bmx cytosolic tyrosine kinase/ETKcytoplasmic tyrosine-protein kinase BMX/NTK38/NTK38 tyrosine kinase/PSCTK2; BMX elisa kit

• Reactivity: Human
• Sequence Length: 675

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.56-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for BMX elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human BMX antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human BMX antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human BMX amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human BMX can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 42544182
• NCBI GeneID: 660
• NCBI Accession #: NP_975010.1
• NCBI GenBank Nucleotide #: NM_203281.2
• Molecular Weight: 78,011 Da
• NCBI Official Full Name: cytoplasmic tyrosine-protein kinase BMX
• NCBI Official Synonym Full Names: BMX non-receptor tyrosine kinase
• NCBI Official Symbol: BMX
• NCBI Official Synonym Symbols: ETK; PSCTK2; PSCTK3
• NCBI Protein Information: cytoplasmic tyrosine-protein kinase BMX
• UniProt Protein Name: Cytoplasmic tyrosine-protein kinase BMX
• Protein Family: Cytoplasmic tyrosine-protein kinase
• UniProt Gene Name: BMX
• UniProt Synonym Gene Names: ETK
• UniProt Entry Name: BMX_HUMAN

NCBI Description

This gene encodes a non-receptor tyrosine kinase belonging to the Tec kinase family. The protein contains a PH-like domain, which mediates membrane targeting by binding to phosphatidylinositol 3,4,5-triphosphate (PIP3), and a SH2 domain that binds to tyrosine-phosphorylated proteins and functions in signal transduction. The protein is implicated in several signal transduction pathways including the Stat pathway, and regulates differentiation and tumorigenicity of several types of cancer cells. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Sep 2009]

Uniprot Description

Etk: a tyrosine kinase of the Tec family. Activity is required for IL6-induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.

Protein type: Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, TK; EC 2.7.10.2; TK group; Tec family

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: cytosol; extrinsic to internal side of plasma membrane

Molecular Function: ATP binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein-tyrosine kinase activity; receptor binding; signal transducer activity

Biological Process: apoptosis; cell structure disassembly during apoptosis; innate immune response; mesoderm development; NK T cell differentiation; programmed cell death; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway