Rabbit anti-Human, Mouse PRMT1 Polyclonal Antibody | anti-PRMT1 antibody

PRMT1 (Protein arginine N Methyltransferase 1, ANM1, HCP1, HRMT1L2, IR1B4) (MaxLight 550)

Cat. Num. AAA6494332

• Gene Names: PRMT1; ANM1; HCP1; IR1B4; HRMT1L2
• Reactivity: Human, Mouse
• Applications: Immunoblot, Western Blot, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRMT1; Polyclonal Antibody; PRMT1 (Protein arginine N Methyltransferase 1; ANM1; HCP1; HRMT1L2; IR1B4) (MaxLight 550); anti-PRMT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognize the PRMT1 doublet, Mr ~42-44kD. A non-specific protein was also detected, Mr ~120kD. Species Crossreactivity: Human and mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
• Sequence Length: 1450

• Applicable Applications for anti-PRMT1 antibody: Immunoblot (IB), Western Blot (WB)
• Application Notes: IB: 0.1-0.5ug/ml of this lot detected PRMT1 in RIPA lysates from Jurkat and 3T3/A31, NIH 3T3, HeLa, and mouse ES cells.; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated, synthetic peptide corresponding to aa298-318 (KTGEEIFGTIGMRPNAKNNRD) of human protein arginine methyltransferase 1 (accession number CAA71765). The immunizing sequence is identical in mouse.
• Conjugate: MaxLight550
• Included Positive Antigen Control: Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PRMT1 (0.1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PRMT1 (42kD).)

Product Categories/Family for anti-PRMT1 antibody

Antibodies; Enzymes; Methyltransferase

References

1. Cote J., et. al., Mol. Bio. Cell 14: 274-287 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 359338974
• NCBI GeneID: 3276
• NCBI Accession #: NM_001536.5
• NCBI GenBank Nucleotide #: NM_001536
• UniProt Accession #: Q99873
• Molecular Weight: 4244
• NCBI Official Full Name: Homo sapiens protein arginine methyltransferase 1 (PRMT1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: protein arginine methyltransferase 1
• NCBI Official Symbol: PRMT1
• NCBI Official Synonym Symbols: ANM1; HCP1; IR1B4; HRMT1L2
• NCBI Protein Information: protein arginine N-methyltransferase 1
• UniProt Protein Name: Protein arginine N-methyltransferase 1
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT1
• UniProt Synonym Gene Names: HMT2; HRMT1L2; IR1B4
• UniProt Entry Name: ANM1_HUMAN

NCBI Description

This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

Uniprot Description

PRMT1: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Homodimer and heterodimer with PRMT8. The dimer can then associate to form a homohexamer. Interacts with ILF3, BTG1, BTG2, SUPT5H and interferon-alpha/beta receptor 1. Interacts with NFATC2IP. Widely expressed. By BTG1, BTG2 and ILF3. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.125; Nuclear receptor co-regulator; Methyltransferase, protein arginine

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein-arginine omega-N asymmetric methyltransferase activity; histone methyltransferase activity; methyltransferase activity; protein binding; N-methyltransferase activity

Biological Process: histone methylation; peptidyl-arginine methylation; establishment and/or maintenance of chromatin architecture; cell surface receptor linked signal transduction; protein amino acid methylation; regulation of transcription, DNA-dependent; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of megakaryocyte differentiation; neurite development