Rabbit anti-Human PRMT1 Polyclonal Antibody | anti-PRMT1 antibody

PRMT1, CT (Protein Arginine N-Methyltransferase 1, Interferon Receptor 1-bound Protein 4, HMT2, HRMT1L2, IR1B4)

Cat. Num. AAA624622

• Gene Names: PRMT1; ANM1; HCP1; IR1B4; HRMT1L2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PRMT1; Polyclonal Antibody; CT (Protein Arginine N-Methyltransferase 1; Interferon Receptor 1-bound Protein 4; HMT2; HRMT1L2; IR1B4); Anti -PRMT1; anti-PRMT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRMT1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PRMT1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human PRMT1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PRMT1 (arrow) using rabbit polyclonal PRMT1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT1 gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PRMT1 antibody

Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

Product Categories/Family for anti-PRMT1 antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 333360913
• NCBI GeneID: 3276
• NCBI Accession #: NP_001193971.1
• NCBI GenBank Nucleotide #: NM_001207042.1
• UniProt Accession #: Q5U8W9; Q8WUW5; Q99873
• Molecular Weight: 37,557 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 1 isoform 4
• NCBI Official Synonym Full Names: protein arginine methyltransferase 1
• NCBI Official Symbol: PRMT1
• NCBI Official Synonym Symbols: ANM1; HCP1; IR1B4; HRMT1L2
• NCBI Protein Information: protein arginine N-methyltransferase 1; OTTHUMP00000236172; OTTHUMP00000236176; OTTHUMP00000236178; OTTHUMP00000236179; HMT1 hnRNP methyltransferase-like 2; interferon receptor 1-bound protein 4; histone-arginine N-methyltransferase PRMT1; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2
• UniProt Protein Name: Protein arginine methyltransferase 1 isoform 4
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: HRMT1L2
• UniProt Entry Name: Q5U8W9_HUMAN

NCBI Description

The PRMT1 gene encodes a protein arginine methyltransferase that functions as a histone methyltransferase specific for H4 (see MIM 602822).[supplied by OMIM]

Uniprot Description

PRMT1: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Homodimer and heterodimer with PRMT8. The dimer can then associate to form a homohexamer. Interacts with ILF3, BTG1, BTG2, SUPT5H and interferon-alpha/beta receptor 1. Interacts with NFATC2IP. Widely expressed. By BTG1, BTG2 and ILF3. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Methyltransferase, protein arginine; EC 2.1.1.125; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; histone methyltransferase activity; identical protein binding; protein binding; N-methyltransferase activity

Biological Process: histone methylation; peptidyl-arginine methylation; establishment and/or maintenance of chromatin architecture; cell surface receptor linked signal transduction; protein amino acid methylation; regulation of transcription, DNA-dependent; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; neurite development; negative regulation of megakaryocyte differentiation