Rabbit PRMT-5 Polyclonal Antibody | anti-PRMT-5 antibody

PRMT-5 Antibody

Cat. Num. AAA840578

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: PRMT-5; Polyclonal Antibody; PRMT-5 Antibody; PRMT5; Protein Arginine Methyltransferase 5; HRMT1L5; HMT1 hnRNP Methyltransferase-Like 5; SKB1; Shk1 Kinase-Binding protein; IBP72; 72 kDa ICln-binding protein; JBP1; Jak-binding protein 1; anti-PRMT-5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-PRMT-5 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 734

• Applicable Applications for anti-PRMT-5 antibody: Western Blot (WB)
• Application Notes: Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control.
• Immunogen: Synthetic peptide surrounding amino acid 51 of rat PRMT-5
• Positive Control: Jurkat cell lysate
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-PRMT-5 antibody

Background: Protein arginine N-methyltransferase 5 (PRMT-5) is a member of the PRMT family that localizes to the cytoplasm in many types of tissues. At least three types of PRMT enzymes have been identified that shows essential regulatory functions by methylation of key proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 17552468
• NCBI GeneID: 175717
• NCBI Accession #: NP_498112.1
• NCBI GenBank Nucleotide #: NM_065711.6
• Molecular Weight: 83,292 Da
• NCBI Official Full Name: PRMT-5
• NCBI Official Symbol: prmt-5
• NCBI Protein Information: PRMT-5
• UniProt Protein Name: Protein arginine N-methyltransferase 5
• UniProt Gene Name: prmt-5
• UniProt Entry Name: ANM5_CAEEL

Uniprot Description

Catalyzes the symmetrical dimethylation of arginine residues in targets such as small nuclear ribonucleoproteins, histone H2A/H4 and cbp-1 (PubMed:19521535, PubMed:23866019, PubMed:22143770). Dimethylation occurs in a distributive manner where the protein is released after the addition of the first methyl group prior to rebinding for the addition of the second methyl group (PubMed:23866019). Plays a role in the negative regulation of DNA damage-induced apoptosis (PubMed:19521535). By methylating cbp-1, may prevent apoptosis by repressing the capacity of cbp-1 to enhance cep-1 dependent transcription activation of the programmed cell death activator egl-1 (PubMed:19521535).