Rabbit anti-Human, Mouse PRL3 Polyclonal Antibody | anti-PRL3 antibody

PRL3 (PTP4A3, PRL-3, PRL-R, Protein Tyrosine Phosphatase Type IVA, Member 3, Protein-tyrosine Phosphatase 4a3, Protein-tyrosine Phosphatase Of Regenerating Liver 3) (Biotin)

Cat. Num. AAA6495864

• Gene Names: PTP4A3; PRL3; PRL-3; PRL-R
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: PRL3; Polyclonal Antibody; PRL3 (PTP4A3; PRL-3; PRL-R; Protein Tyrosine Phosphatase Type IVA; Member 3; Protein-tyrosine Phosphatase 4a3; Protein-tyrosine Phosphatase Of Regenerating Liver 3) (Biotin); anti-PRL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRL3. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

• Applicable Applications for anti-PRL3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein encoding full length of human PRL3 (NP_116000; Q75365).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse heart tissue lysates (35ug/lane) usingMBS627520. PRL3 (arrow) was detected using the purified Pab.)

Related Product Information for anti-PRL3 antibody

The protein encoded by this gene belongs to a small class of prenylated protein tyrosine phosphatases (PTPs). PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. This class of PTPs contain a PTP domain and a characteristic C-terminal prenylation motif. Studies of this class of PTPs in mice demonstrated that they were prenylated proteins in vivo, which suggested their association with cell plasma membrane. Overexpression of this gene in mammalian cells was reported to inhibit angiotensin-II induced cell calcium mobilization and promote cell growth. Two alternatively spliced variants exist. PRL-3 expression was found to be up-regulated in human liver carcinoma compared with normal liver. Its expression in colorectal cancers may contribute to the establishment of liver metastasis. In a different study, PRL-3 is found to be expressed in tumor metastasis and vasculature, regardless of the tumor source.

Product Categories/Family for anti-PRL3 antibody

Antibodies; Enzymes; Phosphatase

References

1. Kato, H., et al. Clin. Cancer Res. 10(21), 7318-7328 (2004). 2. Wu, X., et al. Am. J. Pathol. 164(6), 2039-2054 (2004). 3. Kim, K.A., et al. FEBS Lett. 565(1-3), 181-187 (2004). 4. Kozlov, G., et al. J. Biol. Chem. 279(12), 11882-11889 (2004). 5. Bardelli, A., eta l. Clin. Cancer Res. 9(15), 5607-5615 (2003). 6. Saha, S., et al. Science 294(5545), 1343-1346 (2001). 7. Matter, W.F., et al. Biochem. Biophys. Res. Commun. 283(5), 1061-1068 (2001). 8 Zeng, Q., et al. J. Biol. Chem. 275(28), 21444-21452 (2000). 9. Zeng, Q., et al. Biochem. Biophys. Res. Commun. 244(2), 421-427 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 14589856
• NCBI GeneID: 11156
• NCBI Accession #: NP_116000
• NCBI GenBank Nucleotide #: NM_032611.2
• UniProt Accession #: Q75365; Q8IVN5; Q99849; Q9BTW5; O75365
• Molecular Weight: 21.6 kDa (193aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: protein tyrosine phosphatase type IVA 3 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase type IVA, member 3
• NCBI Official Symbol: PTP4A3
• NCBI Official Synonym Symbols: PRL3; PRL-3; PRL-R
• NCBI Protein Information: protein tyrosine phosphatase type IVA 3

NCBI Description

This gene encodes a member of the protein-tyrosine phosphatase family. Protein tyrosine phosphatases are cell signaling molecules that play regulatory roles in a variety of cellular processes. Studies of this class of protein tyrosine phosphatase in mice demonstrates that they are prenylated in vivo, suggesting their association with cell plasma membrane. The encoded protein may enhance cell proliferation, and overexpression of this gene has been implicated in tumor metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]