Rabbit PRKX Polyclonal Antibody | anti-PRKX antibody

PRKX Polyclonal Antibody

Cat. Num. AAA3006104

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PRKX; Polyclonal Antibody; PRKX Polyclonal Antibody; cAMP-dependent protein kinase catalytic subunit PRKX; PrKX; Protein kinase X; Protein kinase X-linked; Serine/threonine-protein kinase PRKX; Protein kinase PKX1; PKX1; anti-PRKX antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PRKX polyclonal antibody detects endogenous levels of PRKX protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 358

• Applicable Applications for anti-PRKX antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 308-353 of Human PRKX.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PRKX polyclonal antibody at 1:500 dilutionLane1:HEK29T cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PRKX pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PRKX pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PRKX pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-PRKX antibody

The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. PRKY (protein kinase, Y-linked) and PRKX (protein kinase, X-linked) are members of the Ser/Thr protein kinase family, both of which belong to the subfamily of cAMP-dependent kinases. Encoded by a gene that is located near the pseudoautosomal region on chromosome Y, PRKY contains one protein kinase domain through which it catalyzes the ATP-dependent phosphorylation of target proteins. Functioning in a similar manner to PRXY, PRKX contains one protein kinase domain and is essential for macrophage differentiation and renal epithelial cell migration. Defects in the genes encoding PRKY and PRXX are associated with sex reversal disorder, namely XX in males and XY in females.

NCBI and Uniprot Product Information

• NCBI GI #: 4826948
• NCBI Accession #: NP_005035.1
• NCBI GenBank Nucleotide #: NP_005035.1
• UniProt Accession #: P51817
• Molecular Weight: ~ 41kDa
• NCBI Official Full Name: cAMP-dependent protein kinase catalytic subunit PRKX
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit PRKX
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKX
• UniProt Synonym Gene Names: PKX1; PrKX; Protein kinase X; Protein kinase X-linked
• UniProt Entry Name: PRKX_HUMAN

Uniprot Description

PRKX: Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation. A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; AGC group; PKA family

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; cAMP-dependent protein kinase activity; ATP binding

Biological Process: regulation of cell adhesion; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; endothelial cell proliferation; endothelial cell migration; myeloid cell differentiation; angiogenesis; cell adhesion; regulation of cell migration; cell-substrate adhesion