Rabbit anti-Human PRKX Polyclonal Antibody | anti-PRKX antibody

PRKX (cAMP-dependent Protein Kinase Catalytic Subunit PRKX, PrKX, Protein Kinase X, Protein Kinase X-linked, Serine/Threonine-protein kinase PRKX, Protein Kinase PKX1, PKX1) APC

Cat. Num. AAA6390792

• Gene Names: PRKX; PKX1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRKX; Polyclonal Antibody; PRKX (cAMP-dependent Protein Kinase Catalytic Subunit PRKX; PrKX; Protein Kinase X; Protein Kinase X-linked; Serine/Threonine-protein kinase PRKX; Protein Kinase PKX1; PKX1) APC; anti-PRKX antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRKX.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PRKX antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PRKX, aa1-358 (NP_005035.1).
• Immunogen Sequence: MEAPGLAQAAAAESDSRKVAEETPDGAPALCPSPEALSPEPPVYSLQDFDTLATVGTGTFGRVHLVKEKTAKHFFALKVMSIPDVIRLKQEQHVHNEKSVLKEVSHPFLIRLFWTWHDERFLYMLMEYVPGGELFSYLRNRGRFSSTTGLFYSAEIICAIEYLHSKEIVYRDLKPENILLDRDGHIKLTDFGFAKKLVDRTWTLCGTPEYLAPEVIQSKGHGRAVDWWALGILIFEMLSGFPPFFDDNPFGIYQKILAGKIDFPRHLDFHVKDLIKKLLVVDRTRRLGNMKNGANDVKHHRWFRSVDWEAVPQRKLKPPIVPKIAGDGDTSNFETYPENDWDTAAPVPQKDLEIFKNF
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PRKX expression in transfected 293T cell line by PRKX polyclonal antibody. Lane 1: PRKX transfected lysate (40.9kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PRKX antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK) The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).

Product Categories/Family for anti-PRKX antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4826948
• NCBI GeneID: 5613
• NCBI Accession #: NP_005035.1
• NCBI GenBank Nucleotide #: NM_005044.1
• UniProt Accession #: P51817
• Molecular Weight: 40,896 Da
• NCBI Official Full Name: cAMP-dependent protein kinase catalytic subunit PRKX
• NCBI Official Synonym Full Names: protein kinase, X-linked
• NCBI Official Symbol: PRKX
• NCBI Official Synonym Symbols: PKX1
• NCBI Protein Information: cAMP-dependent protein kinase catalytic subunit PRKX; protein kinase X; protein kinase PKX1; protein kinase X-linked; serine/threonine-protein kinase PRKX
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit PRKX
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKX
• UniProt Synonym Gene Names: PKX1; PrKX; Protein kinase X; Protein kinase X-linked
• UniProt Entry Name: PRKX_HUMAN

NCBI Description

This gene encodes a serine threonine protein kinase that has similarity to the catalytic subunit of cyclic AMP dependent protein kinases. The encoded protein is developmentally regulated and may be involved in renal epithelial morphogenesis. This protein may also be involved in macrophage and granulocyte maturation. Abnormal recombination between this gene and a related pseudogene on chromosome Y is a frequent cause of sex reversal disorder in XX males and XY females. Pseudogenes of this gene are found on chromosomes X, 15 and Y. [provided by RefSeq, Feb 2010]

Uniprot Description

PRKX: Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation. A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; AGC group; PKA family

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; cAMP-dependent protein kinase activity; ATP binding

Biological Process: regulation of cell adhesion; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; endothelial cell proliferation; endothelial cell migration; myeloid cell differentiation; angiogenesis; cell adhesion; regulation of cell migration; cell-substrate adhesion