Rabbit anti-Human PRKRA Polyclonal Antibody | anti-PRKRA antibody

PRKRA, Phosphorylated (Ser246) (Interferon-inducible Double Stranded RNA-dependent Protein Kinase Activator A, Protein Kinase, Interferon-inducible Double Stranded RNA-dependent Activator, Protein Activator Of The Interferon-induced Protein Kinase, PKR-as

Cat. Num. AAA6495338

• Gene Names: PRKRA; RAX; PACT; DYT16; HSD14
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRKRA; Polyclonal Antibody; Phosphorylated (Ser246) (Interferon-inducible Double Stranded RNA-dependent Protein Kinase Activator A; Protein Kinase; Interferon-inducible Double Stranded RNA-dependent Activator; Protein Activator Of The Interferon-induced Protein Kinase; PKR-as; anti-PRKRA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRKRA when phosphorylated at Ser246.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PRKRA antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser246 of human PRKRA (KLH).
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-PRKRA-pS246 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-PRKRA antibody

Antibodies; Protein Kinases

References

1. Seibler, P., Lancet Neurol 7(5), 380-381 (2008). 2. Kok, K.H., J. Biol. Chem. 282(24), 17649-17657 (2007). 3. Patel, R.C., EMBO J. 17(15), 4379-4390 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4505581
• NCBI GeneID: 8575
• NCBI Accession #: NP_003681
• NCBI GenBank Nucleotide #: NM_003690.4
• UniProt Accession #: O75569; Q53G24; Q6X7T5; Q8NDK4; A8K3I6
• Molecular Weight: 36.8 kDa (336aa) confirmed by MALDI-TOF
• NCBI Official Full Name: interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 1
• NCBI Official Synonym Full Names: protein activator of interferon induced protein kinase EIF2AK2
• NCBI Official Symbol: PRKRA
• NCBI Official Synonym Symbols: RAX; PACT; DYT16; HSD14
• NCBI Protein Information: interferon-inducible double-stranded RNA-dependent protein kinase activator A
• UniProt Protein Name: Interferon-inducible double-stranded RNA-dependent protein kinase activator A
• Protein Family: Interferon-inducible double-stranded RNA-dependent protein kinase activator
• UniProt Gene Name: PRKRA
• UniProt Synonym Gene Names: PACT; RAX
• UniProt Entry Name: PRKRA_HUMAN

NCBI Description

This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

PACT: a double strand RNA binding protein (dsRBP). Interacts with TRBP and Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. Activates the interferon-induced protein kinase PKR. Three alternatively spliced human isoforms have been reported.

Protein type: Activator; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: cytoplasm; cytosol; membrane; nucleoplasm

Molecular Function: double-stranded RNA binding; enzyme binding; identical protein binding; protein binding; protein homodimerization activity

Biological Process: immune response; miRNA-mediated gene silencing, production of miRNAs; negative regulation of cell proliferation; pre-microRNA processing; response to virus; RNA interference, production of siRNA; RNA-mediated gene silencing

Disease: Dystonia 16