Rabbit anti-Mouse Prkcb Polyclonal Antibody | anti-Prkcb antibody

Prkcb, NT (Prkcb, Pkcb, Prkcb1, Protein kinase C beta type) (APC)

Cat. Num. AAA6337315

• Gene Names: Prkcb; Pkcb; Prkcb1; Prkcb2; PKC-Beta; A130082F03Rik
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Prkcb; Polyclonal Antibody; NT (Prkcb; Pkcb; Prkcb1; Protein kinase C beta type) (APC); Protein kinase C beta type; anti-Prkcb antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Prkcb antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Prkcb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of Mouse Prkcb.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Prkcb Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the Prkcb antibody detected the Prkcb protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Prkcb Antibody (N-term) (AP14697a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Prkcb Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-Prkcb antibody

Antibodies; Protein Kinases

References

Yamamoto, T., et al. J. Lipid Res. 51(7):1859-1870(2010). Deng, P.Y., et al. J. Neurosci. 30(15):5136-5148(2010). Hennige, A.M., et al. J. Cell. Mol. Med. 14(4):903-913(2010). Kim, Y.A., et al. Neurosci. Lett. 468(3):254-258(2010). Kleiman, E., et al. Biochem. Biophys. Res. Commun. 388(3):554-559(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 6679345
• NCBI GeneID: 18751
• NCBI Accession #: NP_032881.1
• NCBI GenBank Nucleotide #: NM_008855.2
• UniProt Accession #: P68404; P04410; P04411; A0JNZ5; F2Z441
• Molecular Weight: 76,751 Da
• NCBI Official Full Name: protein kinase C beta type
• NCBI Official Synonym Full Names: protein kinase C, beta
• NCBI Official Symbol: Prkcb
• NCBI Official Synonym Symbols: Pkcb; Prkcb1; Prkcb2; PKC-Beta; A130082F03Rik
• NCBI Protein Information: protein kinase C beta type; PKC-B; protein kinase C beta-II; protein kinase C, beta 1
• UniProt Protein Name: Protein kinase C beta type
• Protein Family: Protein kinase
• UniProt Gene Name: Prkcb
• UniProt Synonym Gene Names: Pkcb; Prkcb1; PKC-B; PKC-beta
• UniProt Entry Name: KPCB_MOUSE

Uniprot Description

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription ().