Heat Shock Transcription Factor 4 Recombinant Protein | HSF4 recombinant protein

Recombinant Heat Shock Transcription Factor 4 (HSF4)

Cat. Num. AAA2030480

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Heat Shock Transcription Factor 4; Recombinant Heat Shock Transcription Factor 4 (HSF4); HSF4 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; VVTLRQSHGQ QHRVIGKLIQ CLFGPLQAGP SNAGGKRKLS LMLDEGSSCP TPAKFNTCPL PGALLQDPYF IQSPLPETNL GLSPHRARGP IISDIPEDSP SPEGTRLSPS SDGRSPPATACGCG AGHPGREREL QPRGAQECPT A
• Sequence Length: 463

• Applicable Applications for HSF4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 45.2kDa
• Accurate Molecular Mass (KD): 44kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, GST-tag, S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ser19~Lys179
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 1813426
• Molecular Weight: 49,952 Da
• NCBI Official Full Name: heat shock transcription factor 4
• UniProt Protein Name: Heat shock factor protein 4
• Protein Family: Heat shock factor protein
• UniProt Gene Name: HSF4
• UniProt Synonym Gene Names: HSF 4; hHSF4; HSTF 4
• UniProt Entry Name: HSF4_HUMAN

Uniprot Description

HSF4: DNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription. Defects in HSF4 are the cause of cataract, zonular (CZ). A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes. Defects in HSF4 are the cause of cataract Marner type (CAM). A form of cataract with variable and progressive opacities. Affected individuals present with zonular cataract, although some have nuclear, anterior polar, or stellate cataract. Finger malformation is observed in some kindreds. Belongs to the HSF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Cell development/differentiation; DNA-binding

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: nucleoplasm

Molecular Function: sequence-specific DNA binding; transcription factor activity; protein phosphatase binding; transcription corepressor activity

Biological Process: histone H3-K9 demethylation; camera-type eye development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; cell development

Disease: Cataract 5, Multiple Types