Human Insulin Like Growth Factor 2 Receptor ELISA Kit | IGF2R elisa kit

Human Insulin Like Growth Factor 2 Receptor ELISA Kit

Cat. Num. AAA764369

• Gene Names: IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R; MPR 300; M6P/IGF2R
• Reactivity: Human
• Synonyms: Insulin Like Growth Factor 2 Receptor; Human Insulin Like Growth Factor 2 Receptor ELISA Kit; IGF2R/CD222/CIMPR/M6P-R/MPR1/MPRI/cation-independent mannose-6-phosphate receptor/Cation-independent mannose-6-phosphate receptor (CI Man-6-P receptor/CI-MPR/M6PR)/M6P/IGF2 receptor (M6P/IGF2R)/Insulin-like growth factor II receptor (IGF-II receptor)/300 kDa mannose 6-phosphate receptor (MPR 300)/Insulin-like growth factor 2 receptor; IGF2R elisa kit

• Reactivity: Human
• Sequence Length: 2491

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for IGF2R elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human IGF2R antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human IGF2R antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IGF2R amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human IGF2R can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6561206
• NCBI GeneID: 3482
• Molecular Weight: 274,375 Da
• NCBI Official Full Name: insulin-like growth factor 2 receptor
• NCBI Official Synonym Full Names: insulin like growth factor 2 receptor
• NCBI Official Symbol: IGF2R
• NCBI Official Synonym Symbols: MPR1; MPRI; CD222; CIMPR; M6P-R; MPR 300; M6P/IGF2R
• NCBI Protein Information: cation-independent mannose-6-phosphate receptor
• UniProt Protein Name: Cation-independent mannose-6-phosphate receptor
• Protein Family: Cation-independent mannose-6-phosphate receptor
• UniProt Gene Name: IGF2R
• UniProt Synonym Gene Names: MPRI; CI Man-6-P receptor; CI-MPR; M6PR; MPR 300; IGF-II receptor; M6P/IGF2R
• UniProt Entry Name: MPRI_HUMAN

NCBI Description

This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611). [provided by RefSeq, Nov 2015]

Uniprot Description

IGF2R: a multifunctional type I transmembrane protein receptor that binds insulin-like growth factor 2 at the cell surface and mannose-6-phosphate-tagged proteins in the trans-Golgi network. Also known as the cation-independent mannose-6 phosphate (M6P) receptor. Clears IGF2 from the cell surface to attenuate signaling, and transports M6P-tagged lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. M6P-tagged lysosomal enzymes bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Belongs to the MRL1/IGF2R family.

Protein type: Vesicle; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: cell surface; clathrin coat; early endosome; endocytic vesicle; endosome; extracellular space; focal adhesion; integral to plasma membrane; late endosome; lysosomal membrane; membrane; nuclear envelope lumen; perinuclear region of cytoplasm; trans-Golgi network; trans-Golgi network transport vesicle

Molecular Function: enzyme binding; G-protein alpha-subunit binding; G-protein coupled receptor activity; glycoprotein binding; identical protein binding; insulin-like growth factor II binding; insulin-like growth factor receptor activity; mannose binding; phosphoprotein binding; protein binding; receptor activity; retinoic acid binding; transporter activity

Biological Process: G-protein coupled receptor protein signaling pathway; insulin-like growth factor receptor signaling pathway; liver development; organ regeneration; positive regulation of apoptosis; post-embryonic development; receptor-mediated endocytosis; response to retinoic acid; signal transduction; spermatogenesis

Disease: Hepatocellular Carcinoma