Rabbit anti-Human PRKAG2 Polyclonal Antibody | anti-PRKAG2 antibody

PRKAG2 (5'-AMP-activated Protein Kinase Subunit gamma-2, AMPK gamma2, AMPK Subunit gamma-2, H91620p,CMH6, H91620p, WPWS) (AP)

Cat. Num. AAA6390659

• Gene Names: PRKAG2; AAKG; CMH6; WPWS; AAKG2; H91620p
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRKAG2; Polyclonal Antibody; PRKAG2 (5'-AMP-activated Protein Kinase Subunit gamma-2; AMPK gamma2; AMPK Subunit gamma-2; H91620p; CMH6; WPWS) (AP); anti-PRKAG2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRKAG2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 328

• Applicable Applications for anti-PRKAG2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PRKAG2, aa1-328 (NP_077747.1).
• Immunogen Sequence: MLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETE
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PRKAG2 expression in transfected 293T cell line by PRKAG2 polyclonal antibody. Lane 1: PRKAG2 transfected lysate (37.5kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-PRKAG2 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 100913189
• NCBI GeneID: 51422
• NCBI Accession #: NP_077747.1
• NCBI GenBank Nucleotide #: NM_024429
• UniProt Accession #: Q9UGJ0
• NCBI Official Full Name: 5'-AMP-activated protein kinase subunit gamma-2 isoform b
• NCBI Official Synonym Full Names: protein kinase AMP-activated non-catalytic subunit gamma 2
• NCBI Official Symbol: PRKAG2
• NCBI Official Synonym Symbols: AAKG; CMH6; WPWS; AAKG2; H91620p
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit gamma-2
• UniProt Protein Name: 5'-AMP-activated protein kinase subunit gamma-2
• Protein Family: 5'-AMP-activated protein kinase
• UniProt Gene Name: PRKAG2
• UniProt Synonym Gene Names: AMPK gamma2
• UniProt Entry Name: AAKG2_HUMAN

NCBI Description

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2015]

Uniprot Description

AMPKG2: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non- catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2. Isoform B is ubiquitously expressed except in liver and thymus. The highest level is detected in heart with abundant expression in placenta and testis. Belongs to the 5'-AMP-activated protein kinase gamma subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: nucleoplasm; extracellular space; cytosol; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; phosphorylase kinase regulator activity; ADP binding; protein kinase activator activity; protein kinase binding; ATP binding; AMP binding

Biological Process: glycogen metabolic process; mitochondrion organization and biogenesis; organelle organization and biogenesis; carnitine shuttle; cellular lipid metabolic process; protein amino acid phosphorylation; regulation of fatty acid biosynthetic process; ATP biosynthetic process; positive regulation of protein kinase activity; sterol biosynthetic process; regulation of glycolysis; regulation of fatty acid metabolic process; regulation of fatty acid oxidation; energy reserve metabolic process; insulin receptor signaling pathway; negative regulation of protein kinase activity; regulation of glucose import; cell cycle arrest; fatty acid biosynthetic process

Disease: Glycogen Storage Disease Of Heart, Lethal Congenital; Cardiomyopathy, Familial Hypertrophic, 6; Wolff-parkinson-white Syndrome