Rabbit anti-Human, Mouse PRKAG1 Polyclonal Antibody | anti-PRKAG1 antibody

PRKAG1, NT (PRKAG1, 5'-AMP-activated protein kinase subunit gamma-1) (AP)

Cat. Num. AAA6337248

• Gene Names: PRKAG1; AMPKG
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRKAG1; Polyclonal Antibody; NT (PRKAG1; 5'-AMP-activated protein kinase subunit gamma-1) (AP); 5'-AMP-activated protein kinase subunit gamma-1; anti-PRKAG1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 331

• Applicable Applications for anti-PRKAG1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PRKAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human PRKAG1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PRKAG1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PRKAG1 antibody detected the PRKAG1 protein (arrow).)

Western Blot (WB)

(PRKAG1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the PRKAG1 antibody detected the PRKAG1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of PRKAG1 (arrow) using rabbit polyclonal PRKAG1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAG1 gene.)

Related Product Information for anti-PRKAG1 antibody

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product Categories/Family for anti-PRKAG1 antibody

Antibodies; Protein Kinases

References

Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Jassim, G., et al. Pharmacopsychiatry (2010). In press: Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). McGeachie, M., et al. Circulation 120(24):2448-2454(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4506061
• NCBI GeneID: 5571
• NCBI Accession #: NP_002724.1; NP_997626.1
• NCBI GenBank Nucleotide #: NP_002724.1
• UniProt Accession #: P54619
• NCBI Official Full Name: 5'-AMP-activated protein kinase subunit gamma-1 isoform 1
• NCBI Official Synonym Full Names: protein kinase AMP-activated non-catalytic subunit gamma 1
• NCBI Official Symbol: PRKAG1
• NCBI Official Synonym Symbols: AMPKG
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit gamma-1
• UniProt Protein Name: 5'-AMP-activated protein kinase subunit gamma-1
• Protein Family: 5'-AMP-activated protein kinase
• UniProt Gene Name: PRKAG1
• UniProt Synonym Gene Names: AMPK gamma1; AMPK subunit gamma-1; AMPKg
• UniProt Entry Name: AAKG1_HUMAN

NCBI Description

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

AMPKG1: a non-catalytic subunit of AMPK, a highly conserved kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma). Each subunit exists as multiple genetic isoforms giving rise to 12 different possible combinations of holoenzyme. Plays a key role in regulation of energy homeostasis. Activated by an elevated AMP-ATP ratio due to environmental stress, such as heat shock, hypoxia and ischemia. AMPK phosphorylates and regulates many metabolic enzymes.

Protein type: Protein kinase, regulatory subunit; Autophagy

Chromosomal Location of Human Ortholog: 12q12-q14

Cellular Component: membrane; nucleus; cytosol; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; protein binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase regulator activity; ADP binding; protein kinase binding; ATP binding; protein kinase activity; AMP binding

Biological Process: mitochondrion organization and biogenesis; protein heterooligomerization; organelle organization and biogenesis; positive regulation of protein kinase activity; regulation of glycolysis; insulin receptor signaling pathway; spermatogenesis; cell cycle arrest; signal transduction; protein amino acid phosphorylation; fatty acid biosynthetic process