Rabbit PRKAG1/2/3 Polyclonal Antibody | anti-PRKAG1/2/3 antibody

PRKAG1/2/3 Antibody

Cat. Num. AAA9409893

• Gene Names: PRKAG1; AMPKG
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: PRKAG1/2/3; Polyclonal Antibody; PRKAG1/2/3 Antibody; 5'-AMP-activated protein kinase subunit gamma-1; AMPK gamma-1 chain; AMPKg; PRKAG1; anti-PRKAG1/2/3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total PRKAG1/2/3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 340

• Applicable Applications for anti-PRKAG1/2/3 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:3000
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human PRKAG1/2/3.
• Target Name: PRKAG1/2/3
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells and 293 cells, using PRKAG1/2/3 antibody.)

Related Product Information for anti-PRKAG1/2/3 antibody

AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.Gao G., J. Biol. Chem. 271:8675-8681(1996).The MGC Project Team, Genome Res. 14:2121-2127(2004).Baba M., Proc. Natl. Acad. Sci. U.S.A. 103:15552-15557(2006).

Product Categories/Family for anti-PRKAG1/2/3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 332000015
• NCBI GeneID: 5571
• NCBI Accession #: NP_001193638.1
• NCBI GenBank Nucleotide #: NM_001206709.1
• UniProt Accession #: P54619; Q8N7V9; B4DDT7
• Molecular Weight: 38,533 Da
• NCBI Official Full Name: 5'-AMP-activated protein kinase subunit gamma-1 isoform 3
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, gamma 1 non-catalytic subunit
• NCBI Official Symbol: PRKAG1
• NCBI Official Synonym Symbols: AMPKG
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit gamma-1
• UniProt Protein Name: 5'-AMP-activated protein kinase subunit gamma-1
• UniProt Gene Name: PRKAG1
• UniProt Synonym Gene Names: AMPK gamma1; AMPK subunit gamma-1; AMPKg
• UniProt Entry Name: AAKG1_HUMAN

NCBI Description

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

AMPKG1: a non-catalytic subunit of AMPK, a highly conserved kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma). Each subunit exists as multiple genetic isoforms giving rise to 12 different possible combinations of holoenzyme. Plays a key role in regulation of energy homeostasis. Activated by an elevated AMP-ATP ratio due to environmental stress, such as heat shock, hypoxia and ischemia. AMPK phosphorylates and regulates many metabolic enzymes.

Protein type: Autophagy; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 12q12-q14

Cellular Component: membrane; cytosol; nucleus; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; protein binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase regulator activity; ADP binding; protein kinase binding; ATP binding; protein kinase activity; AMP binding

Biological Process: mitochondrion organization and biogenesis; protein heterooligomerization; positive regulation of protein kinase activity; organelle organization and biogenesis; regulation of glycolysis; insulin receptor signaling pathway; spermatogenesis; signal transduction; cell cycle arrest; protein amino acid phosphorylation; fatty acid biosynthetic process