Rabbit anti-Human NFE2 Polyclonal Antibody | anti-NFE2 antibody

NFE2, CT (NFE2, Transcription factor NF-E2 45kD subunit, Leucine zipper protein NF-E2, Nuclear factor, erythroid-derived 2 45kD subunit, p45 NF-E2)

Cat. Num. AAA6003833

• Gene Names: NFE2; p45; NF-E2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NFE2; Polyclonal Antibody; CT (NFE2; Transcription factor NF-E2 45kD subunit; Leucine zipper protein NF-E2; Nuclear factor; erythroid-derived 2 45kD subunit; p45 NF-E2); Anti -NFE2; anti-NFE2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-NFE2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: NFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-340 amino acids from the C-terminal region of human NFE2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NFE2 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the NFE2 antibody detected the NFE2 protein (arrow).)

Related Product Information for anti-NFE2 antibody

Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

Product Categories/Family for anti-NFE2 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 47496515
• NCBI GeneID: 4778
• UniProt Accession #: Q16621; Q07720; Q6ICV9
• Molecular Weight: 41,473 Da
• NCBI Official Full Name: NFE2
• NCBI Official Synonym Full Names: nuclear factor, erythroid 2
• NCBI Official Symbol: NFE2
• NCBI Official Synonym Symbols: p45; NF-E2
• NCBI Protein Information: transcription factor NF-E2 45 kDa subunit; p45 NF-E2; leucine zipper protein NF-E2; nuclear factor (erythroid-derived 2), 45kD; nuclear factor (erythroid-derived 2), 45kDa; nuclear factor, erythroid-derived 2 45 kDa subunit
• UniProt Protein Name: Transcription factor NF-E2 45 kDa subunit
• Protein Family: Protein
• UniProt Gene Name: NFE2
• UniProt Entry Name: NFE2_HUMAN

Uniprot Description

NF-E2: a transcription factor of the bZIP family, CNC subfamily. May play a role in all aspects of hemoglobin production: globin synthesis, heme synthesis, and the procurement of iron. Required for activity at the locus control region (LCR) upstream of the globin gene complexes. Requires p18 NF-E2 for binding to the NF-E2 motif. Expressed in hematopoietic cells and also in colon and testis.

Protein type: Transcription factor; Cytoskeletal; DNA-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; PML body; cytoplasm; nucleus; actin cytoskeleton

Molecular Function: sequence-specific DNA binding; transcription coactivator activity; WW domain binding; protein N-terminus binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; negative regulation of bone mineralization; cell-cell signaling; transcription, DNA-dependent; nucleosome disassembly; blood circulation; hemostasis; multicellular organismal development; positive regulation of transcription, DNA-dependent; blood coagulation