Western Blot (WB)
(Anti-PRKACA rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:44 kDa)
Rabbit anti-Human PRKACA Polyclonal Antibody | anti-PRKACA antibody
Anti-PRKACA Antibody, Rabbit Polyclonal
Gene Names
PRKACA; PKACA
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
PRKACA; Polyclonal Antibody; Anti-PRKACA Antibody; Rabbit Polyclonal; PRKACA Antibody; Rabbit PAb; Antigen Affinity Purified; protein kinase; cAMP-dependent; alpha catalytic subunit; Anti-PKACA Antibody; Anti-PPNAD4 Antibody; anti-PRKACA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PRKACA
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PRKACA antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:100-1:400
IHC-P: 1:100-1:400
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human PRKACA
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PRKACA, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PRKACA rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:44 kDa)
Western Blot (WB)
(Anti-PRKACA rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:44 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human PRKACA in human testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PRKACA in human testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PRKACA in human colon carcinoma with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PRKACA in human colon carcinoma with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-PRKACA antibody
PRKACA encodes a catalytic subunit of protein kinase A, and almost all fibrolamellar carcinomas have a heterozygous 400-kb deletion that leads to the fusion of DNAJB1 and PRKACA. The resulting DNAJB1-PRKACA fusion transcript is believed to activate protein kinase A by dysregulation of the catalytic portion of the protein. In contrast, PRKAR1A encodes one of the regulatory subunits of protein kinase A. Protein kinase A (PRKACA) has been documented as a pivotal regulator in meiosis and mitosis arrest. Somatic mutations of the catalytic subunit of the cyclic AMP-dependent protein kinase (PRKACA) gene have been identified in about 35% of cortisol-producing adenomas (CPAs), with the affected patients showing overt Cushing's syndrome.
Product Categories/Family for anti-PRKACA antibody
References
Nakajima Y, et al. (2014) Somatic mutations of the catalytic subunit of cyclic amp-dependent protein kinase (prkaca) gene in japanese patients with several adrenal adenomas secreting cortisol [rapid communication]. Endocr J 61 (8): 825-832.Graham RP, et al. (2018) Fibrolamellar carcinoma in the carney complex: Prkar1a loss instead of the classic dnajb1-prkaca fusion. Hepatology 68 (4): 1441-1447.Cui C, et al. (2008) Cdc25b acts as a potential target of prkaca in fertilized mouse eggs. Biol Reprod 79 (5): 991-998.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
351
NCBI Official Full Name
cAMP-dependent protein kinase catalytic subunit alpha isoform 1
NCBI Official Synonym Full Names
protein kinase, cAMP-dependent, catalytic, alpha
NCBI Official Symbol
PRKACA
NCBI Official Synonym Symbols
PKACA
NCBI Protein Information
cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; protein kinase A catalytic subunit; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1
UniProt Protein Name
cAMP-dependent protein kinase catalytic subunit alpha
Protein Family
UniProt Gene Name
PRKACA
UniProt Synonym Gene Names
PKACA; PKA C-alpha
UniProt Entry Name
KAPCA_HUMAN
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Product Notes
The PRKACA prkaca (Catalog #AAA8102986) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PRKACA Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRKACA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:100-1:400. Researchers should empirically determine the suitability of the PRKACA prkaca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKACA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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