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Western Blot (WB) (Western blot analysis PRK1/2 (Phospho-Thr774/816) using Na2VO3 treated HepG2 whole cell lysates)

Rabbit anti-Human PRK1/2 Polyclonal Antibody | anti-PRK1/2 antibody

PRK1/2 (Phospho-Thr774/816) Antibody

Gene Names
PKN1; DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
Reactivity
Human
Applications
Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Synonyms
PRK1/2; Polyclonal Antibody; PRK1/2 (Phospho-Thr774/816) Antibody; PKN1; PKN; Protein kinase C-like PKN; Protein kinase N1; PRK1; PRKCL1; Serine-threonine kinase N; Pknalpha; Protease-activated kinase 1; Protein kinase PKN-alpha; DBK; PAK-1; PKN-ALPHA; Protein kinase C-like 1; anti-PRK1/2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
PRK1/2 (Phospho-Thr774/816) Antibody detects endogenous levels of PRK1/2 only when phosphorylated at Thr774/816
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
942
Applicable Applications for anti-PRK1/2 antibody
Western Blot (WB)
Application Notes
Western Blot: 1:1000
Immunogen
A synthesized peptide derived from human PRK1/2 (Phospho-Thr774/816)
Immunogen Type
Peptide
Modification
Phospho-Thr774/816
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis PRK1/2 (Phospho-Thr774/816) using Na2VO3 treated HepG2 whole cell lysates)

Western Blot (WB) (Western blot analysis PRK1/2 (Phospho-Thr774/816) using Na2VO3 treated HepG2 whole cell lysates)
Product Categories/Family for anti-PRK1/2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
66,060 Da
NCBI Official Full Name
serine/threonine-protein kinase N1 isoform 2
NCBI Official Synonym Full Names
protein kinase N1
NCBI Official Symbol
PKN1
NCBI Official Synonym Symbols
DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
NCBI Protein Information
serine/threonine-protein kinase N1
UniProt Protein Name
Serine/threonine-protein kinase N1
UniProt Gene Name
PKN1
UniProt Synonym Gene Names
PAK1; PKN; PRK1; PRKCL1; PAK-1
UniProt Entry Name
PKN1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

PKN1: an AGC kinase of the PKN family. A PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcriptional regulation. Regulates the cytoskeletal network by phosphorylating intermediate filament proteins such as VIM and NFH, NFL and NFM, inhibiting their polymerization. Phosphorylates Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of H3T11, a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3K9me by JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, impairing their import in the nucleus. Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by CASP3 cleavage during apoptosis. Localization to endosomes is mediated via its interaction with RHOB. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. Three isoforms of the human protein are produced by alternative splicing.

Protein type: AGC group; EC 2.7.11.13; Kinase, protein; PKN family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: cleavage furrow; cytoplasm; cytosol; endosome; midbody; nucleoplasm; nucleus

Molecular Function: androgen receptor binding; chromatin binding; GTP-Rho binding; histone binding; histone deacetylase binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein kinase activity; protein kinase C binding; protein serine/threonine kinase activity; Rac GTPase binding

Biological Process: activation of JNK activity; peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of transcription from RNA polymerase II promoter; signal transduction

Research Articles on PRK1/2

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Product Notes

The PRK1/2 pkn1 (Catalog #AAA9416753) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRK1/2 (Phospho-Thr774/816) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRK1/2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1:1000. Researchers should empirically determine the suitability of the PRK1/2 pkn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRK1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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