Rabbit PRICKLE2 Polyclonal Antibody | anti-PRICKLE2 antibody

PRICKLE2 Antibody

Cat. Num. AAA151254

• Gene Names: PRICKLE2; EPM5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: PRICKLE2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PRICKLE2; Polyclonal Antibody; PRICKLE2 Antibody; EPM5; Prickle-like protein 2; prickle homolog 2; anti-PRICKLE2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Two isoforms of PRICKLE2 are known to exist. PRICKLE2 antibody is predicted to not cross-react with other PRICKLE protein family members.
• Purity/Purification: PRICKLE2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 844

• Applicable Applications for anti-PRICKLE2 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: PRICKLE2 antibody can be used for detection of PRICKLE2 by Western blot at 1 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PRICKLE2 antibody was raised against a 15 amino acid synthetic peptide near the center of human PRICKLE2.
• Buffer: PRICKLE2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PRICKLE2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PRICKLE2 in A-20 lysate with PRICKLE2 antibody at 1 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PRICKLE2 in rat brain cells with PRICKLE2 antibody at 20 μg/mL.)

Related Product Information for anti-PRICKLE2 antibody

PRICKLE2 Antibody: PRICKLE2, also known as Pk2 or EPM5, is a member of a highly conserved protein family that function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity. Both PRICKLE2 and the related protein PRICKLE1 are expressed in postmitotic neurons and promote neurite outgrowth, and both proteins promote neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. PRICKLE2 localizes to the postsynaptic density and interacts with PSD-95 and NMDA receptors. Defects in the gene encoding PRICKLE2 are associated with autosomal recessive progressive myoclonic epilepsy.

NCBI and Uniprot Product Information

• NCBI GI #: 38524620
• NCBI GeneID: 166336
• NCBI Accession #: NP_942559
• NCBI GenBank Nucleotide #: NM_198859.3
• UniProt Accession #: Q7Z3G6; Q0VF44
• Molecular Weight: 95,615 Da
• NCBI Official Full Name: prickle-like protein 2
• NCBI Official Synonym Full Names: prickle homolog 2 (Drosophila)
• NCBI Official Symbol: PRICKLE2
• NCBI Official Synonym Symbols: EPM5
• NCBI Protein Information: prickle-like protein 2
• UniProt Protein Name: Prickle-like protein 2
• Protein Family: Prickle-like protein
• UniProt Gene Name: PRICKLE2
• UniProt Entry Name: PRIC2_HUMAN

NCBI Description

This gene encodes a homolog of Drosophila prickle. The exact function of this gene is not known, however, studies in mice suggest that it may be involved in seizure prevention. Mutations in this gene are associated with progressive myoclonic epilepsy type 5. [provided by RefSeq, Dec 2011]

Uniprot Description

PRICKLE2: Defects in PRICKLE2 are the cause of progressive myoclonic epilepsy type 5 (EPM5). EPM5 is a neurodegenerative disorder characterized by myoclonic seizures and variable neurologic symptoms including cognitive decline and persistent movement abnormalities. Belongs to the prickle / espinas / testin family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: nuclear membrane; apicolateral plasma membrane; cytoplasm; lateral plasma membrane

Molecular Function: zinc ion binding

Biological Process: establishment and/or maintenance of epithelial cell polarity; neurite development

Disease: Epilepsy, Progressive Myoclonic 5