Mouse anti-Human Haptoglobin Monoclonal Antibody | anti-HP antibody

Mouse anti Human Haptoglobin:Biotin

Cat. Num. AAA226153

• Gene Names: HP; BP; HPA1S; HP2ALPHA2
• Reactivity: Human
• Applications: ELISA
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: Haptoglobin; Monoclonal Antibody; Mouse anti Human Haptoglobin:Biotin; Haptoglobin antibody | C08-1E10; HAPTOGLOBIN; anti-HP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: C08-1E10
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; Purified IgG conjugated to Biotin - liquid
• Concentration: IgG concentration 0.1mg/ml (varies by lot)

• Applicable Applications for anti-HP antibody: ELISA (EIA)
• Application Notes: ELISA: 1.0ug/ml
• Immunogen: Native haptoglobin purified from human plasma
• Fusion Partners: Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
• Conjugation: Biotin
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.; ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

ELISA (EIA)

(Sandwich ELISA analysis of human haptoglobin using Mouse anti Human haptoglobin as a capture reagent and biotinylated Mouse anti Human haptoglobin (MBS226153) as a detection reagent with purified native human haptoglobin as the antigen. Detecti)

Related Product Information for anti-HP antibody

Mouse anti Human haptoglobin antibody, clone C08-1E10, recognizes haptoglobin, an alpha sialoglycoprotein. Synthesis of haptoglobin is induced by IL-6, IL-1 beta and TNF and it is produced as a pro-protein before it is cleaved in the endoplasmic reticulum by C1r-like protein, to generate the mature form (Redmond et al. 2018). Haptoglobin is composed of two peptide chains referred to as the alpha and beta chains. It is largely expressed by hepatocytes, but is also expressed to a lesser extent in lung, skin and kidney tissues (Vanuytsel et al. 2013). A key role of haptoglobin is its hemoglobin binding capacity, which is utilized during hemolysis to prevent iron loss and subsequent kidney damage. The haptoglobin-hemoglobin complex is removed by the CD163 receptor expressed on the surface of monocytes and macrophages (Sadrzadeh & Bozorgmehr 2004). Haptoglobin also plays roles in the innate and adaptive immune system and has anti-inflammatory activity such as inhibiting monocyte production of TNF, IL-11 and IL-12 (Arredouani et al. 2005).

The biotinylated Mouse anti Human haptoglobin antibody, clone C08-1E10 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human haptoglobin antibody, clone E07-10E9 as the capture antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 386783
• NCBI GeneID: 3240
• UniProt Accession #: P00738; P00737; Q0VAC4; Q2PP15; Q3B7J0; Q6LBY9; Q9UC67; B0AZL5
• Molecular Weight: 45,205 Da
• NCBI Official Full Name: haptoglobin
• NCBI Official Synonym Full Names: haptoglobin
• NCBI Official Symbol: HP
• NCBI Official Synonym Symbols: BP; HPA1S; HP2ALPHA2
• NCBI Protein Information: haptoglobin; zonulin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide
• UniProt Protein Name: Haptoglobin
• Protein Family: Haptoglobin
• UniProt Gene Name: HP
• UniProt Entry Name: HPT_HUMAN

NCBI Description

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: extracellular space; extracellular region

Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity

Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; immune system process; metabolic process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; defense response

Disease: Anhaptoglobinemia