Rabbit anti-Human SOX3 Polyclonal Antibody | anti-SOX3 antibody

SOX3, ID (P204) (SOX3, Transcription factor SOX-3) (MaxLight 550)

Cat. Num. AAA6350026

• Gene Names: SOX3; PHP; GHDX; MRGH; PHPX; SOXB
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: SOX3; Polyclonal Antibody; ID (P204) (SOX3; Transcription factor SOX-3) (MaxLight 550); Transcription factor SOX-3; anti-SOX3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-SOX3 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: SOX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-226 amino acids from the Central region of human SOX3.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SOX3 Antibody (Center P204) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SOX3 antibody detected the SOX3 protein (arrow).)

Flow Cytometry (FC/FACS)

(SOX3 Antibody (Center P204) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SOX3 antibody

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency.

Product Categories/Family for anti-SOX3 antibody

Antibodies; Transcription Factors

References

Dateki, S., et al. J. Clin. Endocrinol. Metab. 95(8):4043-4047(2010). Sato, Y., et al. Mol. Cell. Biochem. 337 (1-2), 267-275 (2010): Mojsin, M., et al. Biochem. J. 425(1):107-116(2010). Bleyl, S.B., et al. Am. J. Med. Genet. A 143A (23), 2785-2795 (2007): Krstic, A., et al. Arch. Biochem. Biophys. 467(2):163-173(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 30061556
• NCBI GeneID: 6658
• NCBI Accession #: NP_005625.2
• NCBI GenBank Nucleotide #: NM_005634.2
• UniProt Accession #: P41225; P35714; Q5JWI3; Q9NP49
• Molecular Weight: 45,210 Da
• NCBI Official Full Name: transcription factor SOX-3
• NCBI Official Synonym Full Names: SRY (sex determining region Y)-box 3
• NCBI Official Symbol: SOX3
• NCBI Official Synonym Symbols: PHP; GHDX; MRGH; PHPX; SOXB
• NCBI Protein Information: transcription factor SOX-3
• UniProt Protein Name: Transcription factor SOX-3
• Protein Family: Transcription factor
• UniProt Gene Name: SOX3
• UniProt Entry Name: SOX3_HUMAN

NCBI Description

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

SOX3: Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. Defects in SOX3 are a cause of panhypopituitarism X- linked (PHPX). Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary. Defects in SOX3 are the cause of mental retardation X- linked with isolated growth hormone deficiency (MRXGH). Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3). A condition in which male gonads develop in a genetic female (female to male sex reversal). Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xq27.1

Cellular Component: nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: sex determination; negative regulation of neuron differentiation; spermatid differentiation; hypothalamus development; organ morphogenesis; central nervous system development; sensory organ development; transcription, DNA-dependent; pituitary gland development; neuron development; negative regulation of transcription from RNA polymerase II promoter; Sertoli cell development

Disease: Panhypopituitarism, X-linked; Mental Retardation, X-linked, With Panhypopituitarism