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Western Blot (WB) (PRB2 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the PRB2 antibody detected PRB2 protein (arrow).)

Rabbit anti-Human, mouse PRB2 Polyclonal Antibody | anti-PRB2 antibody

PRB2 Antibody (C-term)

Gene Names
PRB2; Ps; cP7; IB-9; PRPPRB1
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PRB2; Polyclonal Antibody; PRB2 Antibody (C-term); Basic salivary proline-rich protein 2; Salivary proline-rich protein; Con1 glycoprotein; Basic proline-rich peptide IB-1; Basic proline-rich peptide P-E; IB-9; Basic proline-rich peptide IB-7; Basic proline-rich peptide IB-8c; Basic peptide P-F; Basic proline-rich peptide IB-4; anti-PRB2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 388-416 amino acids from the C-terminal region of human PRB2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
388-416
Sequence Length
416
Applicable Applications for anti-PRB2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(PRB2 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the PRB2 antibody detected PRB2 protein (arrow).)

Western Blot (WB) (PRB2 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the PRB2 antibody detected PRB2 protein (arrow).)

Immunohistochemistry (IHC)

(PRB2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRB2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (PRB2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRB2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(PRB2 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PRB2 antibody detected the PRB2 protein (arrow).)

Western Blot (WB) (PRB2 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PRB2 antibody detected the PRB2 protein (arrow).)

Flow Cytometry (FC/FACS)

(PRB2 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (PRB2 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PRB2 antibody
The function of PRB2 remains unknown.
References
Macaluso, M., et al. Ann. Oncol. 16 SUPPL 4, IV20-IV22 (2005) : Tonini, T., et al. Oncogene 23(28):4930-4937(2004) Azen, E.A., et al. Am. J. Hum. Genet. 58(1):143-153(1996)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
40799
NCBI Official Full Name
Basic salivary proline-rich protein 2
NCBI Official Synonym Full Names
proline-rich protein BstNI subfamily 2
NCBI Official Symbol
PRB2
NCBI Official Synonym Symbols
Ps; cP7; IB-9; PRPPRB1
NCBI Protein Information
basic salivary proline-rich protein 2
UniProt Protein Name
Basic salivary proline-rich protein 2
UniProt Gene Name
PRB2
UniProt Synonym Gene Names
Salivary proline-rich protein
UniProt Entry Name
PRB2_HUMAN

Uniprot Description

PRB2: N- and O-glycosylated. In head and neck cancer patients, O-glycosylated with glucosylgalactosyl carbohydrate moiety. This modification would require prior hydroxylation on the lysine residue. Proteolytically cleaved at the tripeptide Xaa-Pro-Gln, where Xaa in the P3 position is mostly lysine. The endoprotease may be of microbial origin. Pyroglutamate formation occurs on terminal Gln residues of cleaved peptides. Pyroglutamate formation found on at least Gln-398 and Gln-400.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: extracellular region

Research Articles on PRB2

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Product Notes

The PRB2 prb2 (Catalog #AAA9214435) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 388-416. The PRB2 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the PRB2 prb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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